α-dystroglycan Antibody (2237E2D1): sc-65628
- α-dystroglycan Antibody (2237E2D1) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- raised against brain tissue homogenate, with epitope mapping to the glycosylation site of α-dystroglycan of bovine origin
- recommended for detection of brain α-dystroglycan of bovine origin by WB and IP; non cross-reactive with brain α-dystroglycan of mouse, rat and rabbit origin; non cross reactive with skeletal muscle α-dystroglycan
- See α-dystroglycan (IIH6): sc-53987 for α-dystroglycan antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for α-dystroglycan Antibody (2237E2D1) for WB applications. This reagent is now offered in a bundle with α-dystroglycan Antibody (2237E2D1) (see ordering information below).
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α-dystroglycan Antibody (2237E2D1) is a mouse monoclonal IgG2b antibody that detects α-dystroglycan in bovine samples through western blotting (WB) and immunoprecipitation (IP) applications. α-dystroglycan (2237E2D1) antibody recognizes a crucial cell surface receptor that interacts with various extracellular matrix molecules, including laminins, Agrin, and Perlecan, playing a vital role in the formation of basement membranes during early development and the organization of laminin on the cell surface. α-dystroglycan monoclonal antibody (2237E2D1) targets a protein primarily located on the cell membrane, where α-dystroglycan forms a complex with the transmembrane glycoprotein β-dystroglycan, creating the α/β-dystroglycan complex essential for maintaining muscle integrity and function. Anti-α-dystroglycan antibody (2237E2D1) detects a protein integral to the dystrophin-associated protein complex at the sarcolemma and the utrophin-associated protein complex at the neuromuscular junction, facilitating the clustering of nicotinic acetylcholine receptors, which is critical for effective neuromuscular transmission. Proper functioning of α-dystroglycan is essential for muscle cell stability and signaling, making α-dystroglycan a significant target for research in muscle-related diseases and developmental biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
α-dystroglycan Antibody (2237E2D1) References:
- Deficiency of alpha-dystroglycan in muscle-eye-brain disease. | Kano, H., et al. 2002. Biochem Biophys Res Commun. 291: 1283-6. PMID: 11883957
- Aberrant glycosylation of alpha-dystroglycan and congenital muscular dystrophies. | Endo, T. 2005. Acta Myol. 24: 64-9. PMID: 16550917
- Defective glycosylation of α-dystroglycan contributes to podocyte flattening. | Kojima, K., et al. 2011. Kidney Int. 79: 311-6. PMID: 20944549
- Decoding arenavirus pathogenesis: essential roles for alpha-dystroglycan-virus interactions and the immune response. | Oldstone, MB. and Campbell, KP. 2011. Virology. 411: 170-9. PMID: 21185048
- Dissecting the molecular basis of the role of the O-mannosylation pathway in disease: α-dystroglycan and forms of muscular dystrophy. | Live, D., et al. 2013. Chembiochem. 14: 2392-402. PMID: 24318691
- Chemoenzymatic synthesis of α-dystroglycan core M1 O-mannose glycans. | Zhang, Y., et al. 2015. Chem Commun (Camb). 51: 11654-7. PMID: 26100261
- Exogenous expression of the glycosyltransferase LARGE1 restores α-dystroglycan matriglycan and laminin binding in rhabdomyosarcoma. | Beltrán, D., et al. 2019. Skelet Muscle. 9: 11. PMID: 31054580
- HNK-1 sulfotransferase modulates α-dystroglycan glycosylation by 3-O-sulfation of glucuronic acid on matriglycan. | Sheikh, MO., et al. 2020. Glycobiology. 30: 817-829. PMID: 32149355
- Cancer Malignancy Is Correlated with Upregulation of PCYT2-Mediated Glycerol Phosphate Modification of α-Dystroglycan. | Umezawa, F., et al. 2022. Int J Mol Sci. 23: PMID: 35743105
- Identification of Matriglycan by Dual Exoglycosidase Digestion of α-Dystroglycan. | Chandel, I. and Campbell, KP. 2023. Bio Protoc. 13: e4827. PMID: 37753476
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α-dystroglycan Antibody (2237E2D1) | sc-65628 | 200 µg/ml | $316.00 | |||
α-dystroglycan Antibody (2237E2D1): m-IgG Fc BP-HRP Bundle | sc-539186 | 200 µg Ab; 10 µg BP | $354.00 |