Akt3 Double Nickase Plasmid (h): sc-400028-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Akt3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Akt3 Double Nickase Plasmid (h) and Akt3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AKT3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Akt3 Antibody (EE-M14): sc-134254
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Akt3 Double Nickase Plasmid (h) | sc-400028-NIC | 20 µg | $410.00 | |||
Akt3 Double Nickase Plasmid (h2) | sc-400028-NIC-2 | 20 µg | $410.00 |
AKT3 encodes the serine/threonine kinase Akt3, a core effector of PI3K–AKT signaling that integrates growth factor and nutrient cues to regulate cell survival, proliferation, glucose metabolism, and mTOR-linked biosynthetic programs. Akt3 activity influences downstream targets including FOXO transcription factors, GSK3, TSC2, and BAD, shaping apoptosis resistance, cell-cycle progression, and cellular stress responses. In human biology, AKT3 has prominent roles in nervous system development and neuronal homeostasis, and dysregulation of AKT3 signaling has been implicated in cancer-associated signaling rewiring and neurodevelopmental disorders. These pathway connections make AKT3 a useful node for mechanistic studies of signal transduction, lineage-specific growth control, and context-dependent vulnerabilities.
Akt3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AKT3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AKT3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AKT3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AKT3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.