Akt3 Antibody (EE-M14): sc-134254

Akt3 Antibody (EE-M14) is a mouse monoclonal IgG2a kappa light chain antibody that detects Akt3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Akt3 antibody (EE-M14) is available in a non-conjugated form, allowing for versatile experimental use. Akt3, also known as PKBγ or thymoma viral proto-oncogene 3, is a member of the serine/threonine kinase Akt family, which plays a crucial role in various cellular processes, including metabolism, cell survival, and growth. One of the key features of Akt3 is its pleckstrin homology domain, which is essential for activation by phosphoinositides. This domain allows Akt3 to translocate to the plasma membrane upon stimulation by growth factors, where Akt3 becomes phosphorylated and activated. Proper functioning of Akt3 is vital for insulin signaling, as Akt3 mediates glucose uptake and glycogen synthesis in response to insulin. Dysregulation of Akt3 has been implicated in various diseases, including cancer and metabolic disorders, making Akt3 an important target for therapeutic intervention. Using Akt3 monoclonal antibody (EE-M14), researchers can gain insights into Akt3′s role in these critical biological processes and potential implications in health and disease.

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Akt3 Antibody (EE-M14) References:

1. Identification of a human Akt3 (protein kinase B gamma) which contains the regulatory serine phosphorylation site.  |  Nakatani, K., et al. 1999. Biochem Biophys Res Commun. 257: 906-10. PMID: 10208883
2. Mapping of AKT3, encoding a member of the Akt/protein kinase B family, to human and rodent chromosomes by fluorescence in situ hybridization.  |  Murthy, SS., et al. 2000. Cytogenet Cell Genet. 88: 38-40. PMID: 10773662
3. Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction.  |  Burgering, BM. and Coffer, PJ. 1995. Nature. 376: 599-602. PMID: 7637810
4. The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase.  |  Franke, TF., et al. 1995. Cell. 81: 727-36. PMID: 7774014
5. AH/PH domain-mediated interaction between Akt molecules and its potential role in Akt regulation.  |  Datta, K., et al. 1995. Mol Cell Biol. 15: 2304-10. PMID: 7891724
6. Amplification of AKT2 in human pancreatic cells and inhibition of AKT2 expression and tumorigenicity by antisense RNA.  |  Cheng, JQ., et al. 1996. Proc Natl Acad Sci U S A. 93: 3636-41. PMID: 8622988
7. Protein kinase C modulates the insulin-stimulated increase in Akt1 and Akt3 activity in 3T3-L1 adipocytes.  |  Barthel, A., et al. 1998. Biochem Biophys Res Commun. 243: 509-13. PMID: 9480839
8. Akt kinases and 2-deoxyglucose uptake in rat skeletal muscles in vivo: study with insulin and exercise.  |  Turinsky, J. and Damrau-Abney, A. 1999. Am J Physiol. 276: R277-82. PMID: 9887206

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Akt3 Antibody (EE-M14) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-Akt3 Antibody (EE-M14) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Akt3 Antibody (EE-M14) Specifications

Alternate Product Name: Akt3

Product Clone Name: EE-M14

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 100-189

Epitope Target: Akt3

Epitope Species: human

Product Reactivity/Detection: Akt3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for Akt3 Antibody (EE-M14): N/A

The preferred Akt1/2/3 antibody is Akt1/2/3 Antibody (5C10): sc-81434.