Adenosine 5′-[γ-thio]triphosphate tetralithium salt (CAS 93839-89-5)

Adenosine 5′-[γ-thio]triphosphate tetralithium salt is a nucleotide analog that is widely used in molecular biology and biochemistry research. This compound is specifically designed to replace ATP in various enzymatic reactions, where the γ-thio modification provides a sulfur atom in place of an oxygen atom at the terminal phosphate group. This subtle change makes the compound resistant to enzymatic hydrolysis by ATPases, which is a valuable feature for studying the kinetics and mechanisms of phosphorylation-dependent enzymes. Researchers employ adenosine 5′-[γ-thio]triphosphate tetralithium salt in signal transduction studies to probe the function of kinases and other ATP-requiring enzymes by providing a non-hydrolyzable ATP analog. Additionally, it is used in the investigation of motor proteins, such as myosin and kinesin, to understand how ATP hydrolysis is coupled to mechanical work within cells. The tetralithium salt form adds to its solubility in aqueous solutions, facilitating its use in various experimental setups.