ADAMTS-14 Antibody (G-8) recommended for detection of ADAMTS-14 of human origin by WB, IP, IF and ELISA

ADAMTS-14 Antibody (G-8): sc-373773

ADAMTS-14 Antibody (G-8) is rated 4.7 out of 5 by 3.
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Datasheets

    • Anti-ADAMTS-14 Antibody (G-8) is a mouse monoclonal IgG1 (kappa light chain) ADAMTS-14 antibody provided at 200 µg/ml
    • raised against amino acids 1086-1223 mapping at the C-terminus of ADAMTS-14 of human origin
    • ADAMTS-14 Antibody (G-8) is recommended for detection of ADAMTS-14 of human origin by WB, IP, IF and ELISA
    • Anti-ADAMTS-14 Antibody (G-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ADAMTS-14 (G-8): sc-373773.
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What is the recommended starting dilution for Western blot for sc-373773: ADAMTS-14 (G-8) monoclonal antibody?

Asked by: AbPolly
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from 条带单一,非常好 之前申请的试用装,用来做结直肠癌细胞提取蛋白做了westernblot挺好用的。条带单一。推荐使用。
Date published: 2017-07-05
Rated 4 out of 5 by from 条带单一 之前订购了试用装,用作WB,条带明显,符合试验检测要求. 条件1:1000稀释,用作结直肠癌细胞系蛋白的检测
Date published: 2017-03-17
Rated 5 out of 5 by from Good Western blot data of ADAMTS Good Western blot data of ADAMTS-14 expression in non-transfected and human ADAMTS-14 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-11-13
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