Abi-3 Antibody (C-7): sc-376982
- Abi-3 Antibody (C-7) is a mouse monoclonal IgG2a κ Abi-3 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 145-179 within an internal region of Abi-3 of human origin
- Abi-3 Antibody (C-7) is recommended for detection of Abi-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
- Anti-Abi-3 Antibody (C-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Abi-3 Antibody (C-7). This work is in progress.
QUICK LINKS
Abi-3 Antibody (C-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects Abi-3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Abi-3 (C-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Abi-3 protein, also known as NESH, is a 366 amino acid protein that plays a crucial role in cellular signaling pathways by interacting with the Abelson tyrosine kinase (ABL1). This interaction is significant because ABL1 gene encodes a tyrosine kinase essential for mediating mitogenic signaling in response to growth factor receptor activation. The Abi family, which includes Abi-1, Abi-2, and Abi-3, contains SH3-domain proteins that bind to proline-rich motifs of ABL1, thereby activating kinase function. Abi-3 is implicated in the regulation of cell growth and transformation, as well as in the modulation of cell motility through actin polymerization in structures such as lamellipodia and filopodia. Additionally, Abi-3 interacts with TARSH, a gene associated with cellular senescence that can trigger tumor development, highlighting potential role in cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Abi-3 Antibody (C-7) References:
- Isolation and characterization of a novel human gene (NESH) which encodes a putative signaling molecule similar to e3B1 protein. | Miyazaki, K., et al. 2000. Biochim Biophys Acta. 1493: 237-41. PMID: 10978530
- Forced expression of NESH suppresses motility and metastatic dissemination of malignant cells. | Ichigotani, Y., et al. 2002. Cancer Res. 62: 2215-9. PMID: 11956071
- In search of a function for the E3B1/Abi2/Argbp1/NESH family (Review). | Ichigotani, Y., et al. 2002. Int J Mol Med. 9: 591-5. PMID: 12011975
- NESH (Abi-3) is present in the Abi/WAVE complex but does not promote c-Abl-mediated phosphorylation. | Hirao, N., et al. 2006. FEBS Lett. 580: 6464-70. PMID: 17101133
- Insulin receptor substrate protein 53 (IRSp53) as a binding partner of antimetastasis molecule NESH, a member of Abelson interactor protein family. | Matsuda, S., et al. 2008. Ann Oncol. 19: 1356-1357. PMID: 18480067
- Implication of p53-dependent cellular senescence related gene, TARSH in tumor suppression. | Wakoh, T., et al. 2009. Biochem Biophys Res Commun. 380: 807-12. PMID: 19338757
- NESH protein expression switches to the adverse effect of imatinib mesylate. | Matsuda, S., et al. 2008. Mol Oncol. 2: 16-9. PMID: 19383325
- A novel p53-dependent apoptosis function of TARSH in tumor development. | Wakoh, T., et al. 2009. Nagoya J Med Sci. 71: 109-14. PMID: 19994723
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Abi-3 Antibody (C-7) | sc-376982 | 200 µg/ml | $322.00 | |||
Abi-3 Antibody (C-7) AC | sc-376982 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Abi-3 Antibody (C-7) HRP | sc-376982 HRP | 200 µg/ml | $322.00 | |||
Abi-3 Antibody (C-7) FITC | sc-376982 FITC | 200 µg/ml | $336.00 | |||
Abi-3 Antibody (C-7) PE | sc-376982 PE | 200 µg/ml | $349.00 | |||
Abi-3 Antibody (C-7) Alexa Fluor® 488 | sc-376982 AF488 | 200 µg/ml | $364.00 | |||
Abi-3 Antibody (C-7) Alexa Fluor® 546 | sc-376982 AF546 | 200 µg/ml | $364.00 | |||
Abi-3 Antibody (C-7) Alexa Fluor® 594 | sc-376982 AF594 | 200 µg/ml | $364.00 | |||
Abi-3 Antibody (C-7) Alexa Fluor® 647 | sc-376982 AF647 | 200 µg/ml | $364.00 | |||
Abi-3 Antibody (C-7) Alexa Fluor® 680 | sc-376982 AF680 | 200 µg/ml | $364.00 | |||
Abi-3 Antibody (C-7) Alexa Fluor® 790 | sc-376982 AF790 | 200 µg/ml | $364.00 | |||
Abi-3 (C-7) Neutralizing Peptide | sc-376982 P | 100 µg/0.5 ml | $69.00 |