5-Carboxyfluorescein CAS: 76823-03-5
MF: C21H12O7
MW: 376.32
A highly soluble dye which is utilized as a pH indicator.

5-Carboxyfluorescein (CAS 76823-03-5)

5-Carboxyfluorescein | CAS 76823-03-5 is rated 5.0 out of 5 by 1.
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Alternate Names: 5 - FAM
Application: 5-Carboxyfluorescein is a highly soluble dye which is utilized as a pH indicator
CAS Number: 76823-03-5
Molecular Weight: 376.32
Molecular Formula: C21H12O7
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

5-Carboxyfluorescein is a highly soluble dye which is utilized as a pH indicator. 5-Carboxyfluorescein displays strong fluorescence and is concentration dependent. This compound labels proteins, nucleotides and peptides from carboxylic acid interacting with primary amines.


References

1. Weinstein, J.N., et al. 1986. Methods. Enzymol. 128: 657-668. PMID: 3724529
2. David, R., et al. 2003. Biol. Chem. 384: 1619-1630. PMID: 14719805
3. Fischer, R., et al. 2003. Bioconjug. Chem. 14: 653-660. PMID: 12757391

Physical State :
Solid
Solubility :
Soluble in basic aqueous solution (10 mg/ml), DMSO, methanol, and 0.1 N NaOH.
Storage :
Store at -20° C
Boiling Point :
725.2° C at 760 mmHg (Predicted)
Density :
1.7 g/cm3 (Predicted)
Refractive Index :
n20D 1.82 (Predicted)
Fluoresence :
λex 492 nm, λem 518 nm in DMSO
pK Values :
pKa: 6.4
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
123755
MDL Number :
MFCD00036874
Beilstein Registry :
57037
SMILES :
C1=CC2=C(C=C1C(=O)O)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O

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Certificate of Analysis

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I would like to purchase FAM carboxylic acid to obtain dUTP-FAM . Could you please give me a protocol about nucleotide (amino-dUTP) labeling with FAM-carboxylic acid  (not FAM NHS SE)? Thanks

Asked by: HACTGENE
Thank you for your inquiry and your interest in this product. Unfortuantely we do not provide a specific protocol for this application. You might want to check the references provided on this site for any additional information. For any further questions please contact our European Support Office in Germany directly. You can reach us by phone at: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com We are happy to assist you.
Answered by: Technical Support Europe
Date published: 2017-12-19
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Rated 5 out of 5 by from The use of 5 The use of 5-carboxyfluorescein as a soluble dye used as a pH indicator is cited in various publications. -SCBT Publication Review
Date published: 2015-01-22
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