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5-Carboxyfluorescein (CAS 76823-03-5)

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Alternate Names:
5 - FAM
Application:
5-Carboxyfluorescein is a highly soluble dye which is utilized as a pH indicator
CAS Number:
76823-03-5
Molecular Weight:
376.32
Molecular Formula:
C21H12O7
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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5-Carboxyfluorescein is a highly soluble dye which is utilized as a pH indicator. 5-Carboxyfluorescein displays strong fluorescence and is concentration dependent. This compound labels proteins, nucleotides and peptides from carboxylic acid interacting with primary amines.


5-Carboxyfluorescein (CAS 76823-03-5) References

  1. Extending the applicability of carboxyfluorescein in solid-phase synthesis.  |  Fischer, R., et al. 2003. Bioconjug Chem. 14: 653-60. PMID: 12757391
  2. Semisynthesis and application of carboxyfluorescein-labelled biologically active human interleukin-8.  |  David, R., et al. 2003. Biol Chem. 384: 1619-30. PMID: 14719805
  3. 5-Carboxyfluorescein tagged N-phenylanthranilamide as a tracer reagent for fluorescence polarization: a robust method to screen MAPK pathway allosteric inhibitors.  |  Rezvani, ZN., et al. 2010. Chem Commun (Camb). 46: 2043-5. PMID: 20221486
  4. The synthesis and application of Fmoc-Lys(5-Fam) building blocks.  |  Tokmina-Roszyk, M., et al. 2013. Biopolymers. 100: 347-55. PMID: 23444261
  5. Molecular Diffusion through Cyanobacterial Septal Junctions.  |  Nieves-Morión, M., et al. 2017. mBio. 8: PMID: 28049144
  6. Fluorescence Enhancement Using Bimetal Surface Plasmon-Coupled Emission from 5-Carboxyfluorescein (FAM).  |  Tran, NHT., et al. 2018. Micromachines (Basel). 9: PMID: 30424393
  7. Fluorometric methods for determination of H2O2, glucose and cholesterol by using MnO2 nanosheets modified with 5-carboxyfluorescein.  |  Han, T., et al. 2019. Mikrochim Acta. 186: 269. PMID: 30955099
  8. 5-Carboxyfluorescein: intrinsic peroxidase-like catalytic activity and its application in the biomimetic synthesis of polyaniline nanoplatelets.  |  Hu, Q., et al. 2017. J Mater Chem B. 5: 5937-5941. PMID: 32264350
  9. Double-Resonant Nanostructured Gold Surface for Multiplexed Detection.  |  Minopoli, A., et al. 2022. ACS Appl Mater Interfaces. 14: 6417-6427. PMID: 35089707
  10. Determination of Fumonisin B1 by Aptamer-Based Fluorescence Resonance Energy Transfer.  |  Zhao, X., et al. 2022. Sensors (Basel). 22: PMID: 36433193
  11. Identification of 5-Carboxyfluorescein as a Probe Substrate of SLC46A3 and Its Application in a Fluorescence-Based In Vitro Assay Evaluating the Interaction with SLC46A3.  |  Tomabechi, R., et al. 2023. Mol Pharm. 20: 491-499. PMID: 36458938
  12. Carboxyfluorescein leakage assay for lipoprotein-liposome interaction.  |  Weinstein, JN., et al. 1986. Methods Enzymol. 128: 657-68. PMID: 3724529

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

5-Carboxyfluorescein, 100 mg

sc-205903
100 mg
$132.00

5-Carboxyfluorescein, 250 mg

sc-205903A
250 mg
$281.00

I would like to purchase FAM carboxylic acid to obtain dUTP-FAM . Could you please give me a protocol about nucleotide (amino-dUTP) labeling with FAM-carboxylic acid  (not FAM NHS SE)? Thanks

Asked by: HACTGENE
Thank you for your inquiry and your interest in this product. Unfortuantely we do not provide a specific protocol for this application. You might want to check the references provided on this site for any additional information. For any further questions please contact our European Support Office in Germany directly. You can reach us by phone at: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com We are happy to assist you.
Answered by: Technical Support Europe
Date published: 2017-12-19
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Rated 5 out of 5 by from The use of 5The use of 5-carboxyfluorescein as a soluble dye used as a pH indicator is cited in various publications. -SCBT Publication Review
Date published: 2015-01-22
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5-Carboxyfluorescein is rated 5.0 out of 5 by 1.
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