5-Carboxyfluorescein is a highly soluble dye which is utilized as a pH indicator. 5-Carboxyfluorescein displays strong fluorescence and is concentration dependent. This compound labels proteins, nucleotides and peptides from carboxylic acid interacting with primary amines.
1. Weinstein, J.N., et al. 1986. Methods. Enzymol. 128: 657-668. PMID: 3724529 2. David, R., et al. 2003. Biol. Chem. 384: 1619-1630. PMID: 14719805 3. Fischer, R., et al. 2003. Bioconjug. Chem. 14: 653-660. PMID: 12757391
Soluble in basic aqueous solution (10 mg/ml), DMSO, methanol, and 0.1 N NaOH.
Store at -20° C
725.2° C at 760 mmHg (Predicted)
1.7 g/cm3 (Predicted)
n20D 1.82 (Predicted)
λex 492 nm, λem 518 nm in DMSO
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Adobe Acrobat Reader is required to reliably view, print and comment on PDF documents
I would like to purchase FAM carboxylic acid to obtain dUTP-FAM .
Could you please give me a protocol about nucleotide (amino-dUTP) labeling with FAM-carboxylic acid (not FAM NHS SE)?
Asked by: HACTGENE
Thank you for your inquiry and your interest in this product. Unfortuantely we do not provide a specific protocol for this application. You might want to check the references provided on this site for any additional information. For any further questions please contact our European Support Office in Germany directly. You can reach us by phone at: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com We are happy to assist you.
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn