Date published: 2025-10-19

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5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (CAS 117548-22-8)

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Application:
5(6)-Carboxyfluorescein N-hydroxysuccinimide ester is a fluorescent probe of tubulin derivatives
CAS Number:
117548-22-8
Molecular Weight:
473.39
Molecular Formula:
C25H15NO9
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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This product is a fluorescent probe useful for the preparation of tubulin derivatives. It can also be used to determine this product′s own presence in plasma and serum using fluorescence polazrization immunoassay. It has been shown to react with primary and secondary amine groups on proteins and biomolecules, and act as a fluorescent label for these groups.


5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (CAS 117548-22-8) References

  1. Sequential electroelution and mass spectroscopic identification of intact sodium dodecyl sulfate-proteins labeled with 5(6)-carboxyfluorescein-N-hydroxysuccinimide ester.  |  Yefimov, S., et al. 2001. Electrophoresis. 22: 2881-7. PMID: 11565783
  2. Detection of biotinylated proteins in polyacrylamide gels using an avidin-fluorescein conjugate.  |  Nakamura, M., et al. 2002. Anal Biochem. 304: 231-5. PMID: 12009700
  3. Synthesis of adenosine derivatives as transcription initiators and preparation of 5' fluorescein- and biotin-labeled RNA through one-step in vitro transcription.  |  Huang, F., et al. 2003. RNA. 9: 1562-70. PMID: 14624011
  4. Enhancement of intracellular concentration and biological activity of PNA after conjugation with a cell-penetrating synthetic model peptide.  |  Oehlke, J., et al. 2004. Eur J Biochem. 271: 3043-9. PMID: 15233801
  5. Uptake of cell-penetrating peptides is dependent on peptide-to-cell ratio rather than on peptide concentration.  |  Hällbrink, M., et al. 2004. Biochim Biophys Acta. 1667: 222-8. PMID: 15581859
  6. Chemoselective cross-linking and functionalization of alginate via Staudinger ligation.  |  Gattás-Asfura, KM. and Stabler, CL. 2009. Biomacromolecules. 10: 3122-9. PMID: 19848408
  7. Cellular uptake and biological activity of peptide nucleic acids conjugated with peptides with and without cell-penetrating ability.  |  Turner, Y., et al. 2010. J Pept Sci. 16: 71-80. PMID: 19943337
  8. Growth factor- and adhesion protein-like components of fetal calf serum can significantly enhance the intracellular delivery of Peptide nucleic acids.  |  Oehlke, J., et al. 2011. Nucleic Acid Ther. 21: 285-91. PMID: 21851222
  9. Hepatocyte targeting using pegylated asialofetuin-conjugated liposomes.  |  Detampel, P., et al. 2014. J Drug Target. 22: 232-241. PMID: 24328572
  10. A novel method for non-transferrin-bound iron quantification by chelatable fluorescent beads based on flow cytometry.  |  Ma, Y., et al. 2014. Biochem J. 463: 351-62. PMID: 25093426
  11. Hydrophilic fluorescein derivatives: useful reagents for liposome immunolytic assays.  |  Fiechtner, M., et al. 1989. Anal Biochem. 180: 140-6. PMID: 2817337
  12. Comparison of three common amine reactive fluorescent probes used for conjugation to biomolecules by capillary zone electrophoresis.  |  Banks, PR. and Paquette, DM. 1995. Bioconjug Chem. 6: 447-58. PMID: 7578365
  13. On-cell catalysis by surface engineering of live cells with an artificial metalloenzyme  |  Szponarski, M., Schwizer, F., Ward, T. R., & Gademann, K. 2018. Communications Chemistry. 1(1): 84.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester, 100 mg

sc-205993
100 mg
$148.00

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester, 1 g

sc-205993A
1 g
$1224.00