4-Nitrophenyl 2,4,6-Tri-O-acetyl-3-O-(2,3,4,6-tetra-O-acetyl -b-D-glucopyranosyl)-α-D-glucopyranoside

4-Nitrophenyl 2,4,6-Tri-O-acetyl-3-O-(2,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl)-α-D-glucopyranoside is a compound frequently utilized in glycobiology research to investigate carbohydrate-active enzymes (CAZymes) and glycosylation processes. Its structural complexity mimics natural glycosides, making it an ideal substrate for various glycosidases, including β-glucosidases and α-glucosidases. The acetyl groups provide protection to specific hydroxyl functionalities, enabling selective enzymatic hydrolysis by glycosidases. Upon enzymatic cleavage, the 4-nitrophenyl moiety is released, generating a detectable signal that facilitates enzyme activity assays and kinetic studies. Additionally, this compound is employed in the synthesis of glycoconjugates and glycoside derivatives, contributing to the development of carbohydrate-based probes for biological imaging and molecular recognition studies. Furthermore, it aids in elucidating the substrate specificity of glycosidases and their roles in glycan processing pathways. Overall, 4-Nitrophenyl 2,4,6-Tri-O-acetyl-3-O-(2,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl)-α-D-glucopyranoside serves as a valuable tool in glycobiology research, enabling the investigation of glycosylation-related phenomena and enzyme functions at a molecular level.