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4-Methylumbelliferyl β-D-galactopyranoside is a fluorogenic substrate frequently utilized in molecular biology and enzymology research to study β-galactosidase activity. Upon hydrolysis by the enzyme β-galactosidase, it releases a fluorescent product, 4-methylumbelliferone, which can be easily quantified using fluorescence spectrometry. This characteristic makes it useful for monitoring enzyme activity in real-time and for detecting β-galactosidase in various biological systems, including genetically modified organisms where the enzyme is used as a reporter gene. It is also applied in the assessment of microbial contamination in food and pharmaceutical industries, as certain bacteria express β-galactosidase which can be detected using 4-Methylumbelliferyl β-D-galactopyranoside. Moreover, its use extends to the field of histochemistry, where it serves to visualize enzyme activity in tissue sections.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
4-Methylumbelliferyl β-D-galactopyranoside, 250 mg | sc-220945 | 250 mg | $46.00 | |||
4-Methylumbelliferyl β-D-galactopyranoside, 1 g | sc-220945A | 1 g | $123.00 |