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3-(N-Maleimidylpropionyl)biocytin (CAS 102849-12-7)

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Alternate Names:
MPB
Application:
3-(N-Maleimidylpropionyl)biocytin is A useful biotin coupling reagent
CAS Number:
102849-12-7
Purity:
≥95%
Molecular Weight:
523.60
Molecular Formula:
C23H33N5O7S
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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3-(N-Maleimidylpropionyl)biocytin can be used to couple biotin to cystein residues of peptides and proteins. 3-(N-Maleimidopropionyl)-biocytin (MPB) is a highly adaptable reagent that selectively biotinylates thiol groups, making it ideal for thiol-specific labeling. Its specificity for sulfhydryl groups or reduced disulfide bonds enables precise labeling of proteins that possess free sulfhydryl groups within a pH range of 6.5 to 7.5. When combined with avidin- or streptavidin-conjugated markers, such as fluorescent or enzyme-conjugated markers, MPB becomes useful for various applications. It can be utilized to detect protein sulfhydryl groups on dot blots with exceptional sensitivity in the femtomole range. Moreover, MPB finds utility in cell sorting, enzyme immunoassays, protein blotting, and a wide range of cytochemical procedures.


3-(N-Maleimidylpropionyl)biocytin (CAS 102849-12-7) References

  1. A novel labeling approach supports the five-transmembrane model of subunit a of the Escherichia coli ATP synthase.  |  Wada, T., et al. 1999. J Biol Chem. 274: 17353-7. PMID: 10358096
  2. Cryopreservation alters membrane sulfhydryl status of bull spermatozoa: protection by oxidized glutathione.  |  Chatterjee, S., et al. 2001. Mol Reprod Dev. 60: 498-506. PMID: 11746961
  3. Characterization of the first cytoplasmic loop of subunit a of the Escherichia coli ATP synthase by surface labeling, cross-linking, and mutagenesis.  |  Long, JC., et al. 2002. J Biol Chem. 277: 27288-93. PMID: 12021273
  4. Redox control of changes in protein sulfhydryl levels during human sperm capacitation.  |  de Lamirande, E. and Gagnon, C. 2003. Free Radic Biol Med. 35: 1271-85. PMID: 14607526
  5. Membrane topology of system xc- light subunit reveals a re-entrant loop with substrate-restricted accessibility.  |  Gasol, E., et al. 2004. J Biol Chem. 279: 31228-36. PMID: 15151999
  6. Interaction and functional association of protein disulfide isomerase with alphaVbeta3 integrin on endothelial cells.  |  Swiatkowska, M., et al. 2008. FEBS J. 275: 1813-23. PMID: 18331351
  7. Thiols in the alphaIIbbeta3 integrin are necessary for platelet aggregation.  |  Manickam, N., et al. 2008. Br J Haematol. 142: 457-65. PMID: 18537975
  8. Molecular docking-assisted screening reveals tannic acid as a natural protein disulphide isomerase inhibitor with antiplatelet and antithrombotic activities.  |  Ren, L., et al. 2020. J Cell Mol Med. 24: 14257-14269. PMID: 33128352
  9. Localization of cytosolically oriented maleimide-reactive domain of vacuolar H(+)-pyrophosphatase.  |  Zhen, RG., et al. 1994. J Biol Chem. 269: 23342-50. PMID: 8083239
  10. Identifying the physiological electron transfer site of cytochrome c peroxidase by structure-based engineering.  |  Miller, MA., et al. 1996. Biochemistry. 35: 667-73. PMID: 8547245
  11. Enzymatic and biochemical probes of residues external to the translocation pathway of UhpT, the sugar phosphate carrier of Escherichia coli.  |  Matos, M., et al. 1996. J Biol Chem. 271: 18571-5. PMID: 8702506
  12. Membrane topology of subunit a of the F1F0 ATP synthase as determined by labeling of unique cysteine residues.  |  Long, JC., et al. 1998. J Biol Chem. 273: 16235-40. PMID: 9632682
  13. Topology of the region surrounding Glu681 of human AE1 protein, the erythrocyte anion exchanger.  |  Tang, XB., et al. 1998. J Biol Chem. 273: 22545-53. PMID: 9712881
  14. Functional symmetry of UhpT, the sugar phosphate transporter of Escherichia coli.  |  Fann, MC. and Maloney, PC. 1998. J Biol Chem. 273: 33735-40. PMID: 9837961
  15. Redox control of exofacial protein thiols/disulfides by protein disulfide isomerase.  |  Jiang, XM., et al. 1999. J Biol Chem. 274: 2416-23. PMID: 9891011

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

3-(N-Maleimidylpropionyl)biocytin, 50 mg

sc-216373
50 mg
$336.00

3-(N-Maleimidylpropionyl)biocytin, 100 mg

sc-216373A
100 mg
$605.00

what is the solubility of MPB?

Asked by: AHSAN
Thank you for your question. Soluble in DMSO, sparingly soluble in Methanol
Answered by: Ling Chen
Date published: 2024-06-24
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Rated 5 out of 5 by from Various publications cite the use of 3Various publications cite the use of 3-(N-Maleimidylpropionyl)biocytin to couple biotin to cystein residues of peptides and proteins. -SCBT Publication Review
Date published: 2015-01-27
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3-(N-Maleimidylpropionyl)biocytin is rated 5.0 out of 5 by 1.
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