2′-Deoxy-5-hydroxyuridine (CAS 5168-36-5)

2′-Deoxy-5-hydroxyuridine (5-OH-dUrd) is a synthetic nucleoside analogue derived from the natural deoxynucleoside, 2′-deoxyuridine. Over the years, 2′-Deoxy-5-hydroxyuridine has served as a valuable substrate for DNA and RNA synthesis, facilitating the study of enzyme-catalyzed reactions, gene expression, and epigenetic modifications. Moreover, it has emerged as a powerful tool for unraveling the structure and functionality of enzymes and other proteins. In scientific research, 2′-Deoxy-5-hydroxyuridine finds extensive utility. It serves as a substrate for DNA and RNA synthesis, enabling investigations into enzyme-catalyzed reactions, gene expression, and epigenetic modifications. Additionally, it facilitates the exploration of enzyme and protein structure-function relationships. Moreover, it aids in deciphering the mechanism of action of various drugs and sheds light on the role of DNA methylation in gene expression. 2′-Deoxy-5-hydroxyuridine, being a nucleoside analogue, exhibits a modified chemical structure compared to its natural counterpart, 2′-deoxyuridine. This structural alteration allows 2′-Deoxy-5-hydroxyuridine to interact with proteins and enzymes in a distinct manner from 2′-deoxyuridine. For instance, it can bind to DNA polymerase, an enzyme responsible for DNA replication, thereby impeding its activity. This inhibition of DNA polymerase can lead to notable changes in gene expression.