26S Proteasome p42D Antibody (216): sc-65753

26S Proteasome p42D Antibody (216) is a mouse monoclonal IgG1 antibody that detects 26S Proteasome p42D of Drosophila melanogaster origin by western blotting (WB) and immunoprecipitation (IP). Anti-26S Proteasome p42D antibody (216) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The 26S proteasome is a crucial multi-subunit complex responsible for degrading ubiquitinated proteins, a process essential for maintaining cellular homeostasis and regulating various biological pathways. This complex comprises over 30 distinct subunits, including 26S proteasome p42D, which is one of six ATPase base subunits of the 19S regulatory complex. The 19S regulatory complex recognizes ubiquitinated proteins, removes ubiquitin tags, and facilitates protein translocation to the 20S core for degradation. Assembly of the 26S proteasome requires ATP and can be unstable due to size, often disassociating into smaller subcomplexes. 26S proteasome p42D, also known as Rpt4 in yeast and S10b in humans, is integral to protein-chaperone activity of base subunits, linking histone ubiquitylation and methylation, which is critical for transcription initiation and elongation. 26S Proteasome p42D antibody (216) serves as an invaluable tool for researchers studying proteasomal function and regulation in Drosophila melanogaster, providing insights into protein degradation mechanisms and cellular process implications.

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26S Proteasome p42D Antibody (216) References:

1. Assembly of the Drosophila 26 S proteasome is accompanied by extensive subunit rearrangements.  |  Kurucz, E., et al. 2002. Biochem J. 365: 527-36. PMID: 11945175
2. Deletion of proteasomal subunit S5a/Rpn10/p54 causes lethality, multiple mitotic defects and overexpression of proteasomal genes in Drosophila melanogaster.  |  Szlanka, T., et al. 2003. J Cell Sci. 116: 1023-33. PMID: 12584246
3. The HALTED ROOT gene encoding the 26S proteasome subunit RPT2a is essential for the maintenance of Arabidopsis meristems.  |  Ueda, M., et al. 2004. Development. 131: 2101-11. PMID: 15073153
4. ATP hydrolysis-dependent disassembly of the 26S proteasome is part of the catalytic cycle.  |  Babbitt, SE., et al. 2005. Cell. 121: 553-565. PMID: 15907469
5. The proteasome regulatory particle alters the SAGA coactivator to enhance its interactions with transcriptional activators.  |  Lee, D., et al. 2005. Cell. 123: 423-36. PMID: 16269334
6. Mass spectrometric characterization of the affinity-purified human 26S proteasome complex.  |  Wang, X., et al. 2007. Biochemistry. 46: 3553-65. PMID: 17323924
7. Aging perturbs 26S proteasome assembly in Drosophila melanogaster.  |  Vernace, VA., et al. 2007. FASEB J. 21: 2672-82. PMID: 17413001
8. Rpn13p and Rpn14p are involved in the recognition of ubiquitinated Gcn4p by the 26S proteasome.  |  Seong, KM., et al. 2007. FEBS Lett. 581: 2567-73. PMID: 17499717
9. Automated cryoelectron microscopy of 'single particles' applied to the 26S proteasome.  |  Nickell, S., et al. 2007. FEBS Lett. 581: 2751-6. PMID: 17531228
10. A proteasomal ATPase contributes to dislocation of endoplasmic reticulum-associated degradation (ERAD) substrates.  |  Lipson, C., et al. 2008. J Biol Chem. 283: 7166-75. PMID: 18174173
11. An atomic model AAA-ATPase/20S core particle sub-complex of the 26S proteasome.  |  Förster, F., et al. 2009. Biochem Biophys Res Commun. 388: 228-33. PMID: 19653995
12. Localization of the proteasomal ubiquitin receptors Rpn10 and Rpn13 by electron cryomicroscopy.  |  Sakata, E., et al. 2012. Proc Natl Acad Sci U S A. 109: 1479-84. PMID: 22215586

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-26S Proteasome p42D Antibody (216) has 0 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

26S Proteasome p42D Antibody (216) Specifications

Alternate Product Name: 26S Proteasome p42D

Product Clone Name: 216

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: 26S Proteasome

Epitope Species: Drosophila melanogaster

Product Reactivity/Detection: p42D subunit of the 19S regulatory base complex of the 26S Proteasome

Species Reactivity/Detection: d

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for 26S Proteasome p42D Antibody (216): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin