Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
β2C Tubulin CRISPR/Cas9 KO Plasmid (h): sc-400056
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- β2C Tubulin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the β2C Tubulin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: β2C Tubulin Antibody (1A9): sc-134230
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
β2C Tubulin CRISPR/Cas9 KO Plasmid (h) | sc-400056 | 20 µg | $397.00 |
Overview
TUBB4B encodes β2C tubulin, a core component of α/β-tubulin heterodimers that polymerize into microtubules to support cytoskeletal architecture, intracellular transport, and mitotic spindle assembly. Dynamic microtubule remodeling underlies processes including cell cycle progression, organelle positioning, and ciliogenesis, and interfaces with pathways governing polarity and stress responses. Altered tubulin isotype balance and microtubule dynamics are frequently linked to proliferative phenotypes, chromosomal instability, and changes in migratory behavior observed across multiple disease contexts. As a structural and regulatory node in microtubule-dependent biology, β2C tubulin is a useful target for dissecting cytoskeletal control of cell division and trafficking.
β2C Tubulin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TUBB4B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TUBB4B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TUBB4B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish β2C Tubulin protein expression.
This CRISPR knockout system enables efficient generation of TUBB4B-deficient cell models for investigation of β2C Tubulin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TUBB4B exon(s) critical for β2C Tubulin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TUBB4B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by β2C Tubulin CRISPR/Cas9 KO Plasmid (h) and β2C Tubulin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TUBB4B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by β2C Tubulin HDR Plasmid (h) and β2C Tubulin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TUBB4B homology arms to support homology-directed repair at defined TUBB4B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.