Date published: 2025-9-7

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YAP 항체 (H-9): sc-271134

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데이터 시트
  • YAP 항체(H-9) 는 마우스 monoclonal IgG1κ, 70간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 YAP에서 C-terminus에 위치한 379-407의 아미노산사이에 위치한 항원결정부와 특정결합합니다.
  • 안티-YAP 항체 (H-9)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 YAP 를 감지하는 데에 추천한다.
  • YAP 항체(H-9)는 WB(RGB), IF, IHC(P) 및 FCM용으로 Alexa Fluor® 546 또는 Alexa Fluor® 594에 결합된 형태로 제공됩니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-271134 X, 200 µg/0.1 ml)이 있습니다.
  • YAP (H-9): sc-271134무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP 는 YAP 항체 (H-9) 가WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은YAP 항체(H-9) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다)。

빠른 링크

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YAP1 항체(H-9)는 마우스, 쥐 및 인간 유래의 YAP를 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgG1 κ 마우스 모노클로날 YAP 항체입니다. YAP1 항체(H-9)는 공액형 및 비공액형 항-YAP 항체 형태로 제공됩니다. 예스 관련 단백질은 예스(Yes)의 SH3 도메인에 결합하는 능력으로 인해 원래 알려진 14-3-3 결합 분자입니다. YAP와 14-3-3의 결합은 YAP 14-3-3 결합 모티프에 있는 상동 세린 잔기(Ser 112)의 인산화가 필요합니다. 고도로 보존되고 어디에나 존재하는 14-3-3 단백질은 신호 전달에 관여하는 세포 내 인산화 단백질과 결합하여 분화, 세포 주기 진행 및 세포 사멸을 조절합니다. YAP는 14-3-3 단백질에 의해 조절되는 방식으로 원형질막과 세포 골격에서의 이벤트를 핵에서의 전사 억제와 연결할 수 있습니다. YAP는 전사 인자에 존재하는 PPXY 모티프에 결합하여 전사 보조 인자로 작용하는 PDZ 결합 모티프를 가진 전사 보조 인자인 TAZ의 WW 도메인과 상동성을 공유합니다. YAP는 폐, 태반, 전립선, 난소 및 고환에서 높은 수준으로 발현됩니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

YAP 항체 (H-9)

sc-271134
200 µg/ml
$316.00

YAP (H-9): m-IgG Fc BP-HRP 번들

sc-525787
200 µg Ab; 10 µg BP
$354.00

YAP 항체 (H-9) X

sc-271134 X
200 µg/0.1 ml
$316.00

YAP 항체 (H-9) Alexa Fluor® 546

sc-271134 AF546
200 µg/ml
$357.00

YAP 항체 (H-9) Alexa Fluor® 594

sc-271134 AF594
200 µg/ml
$357.00

YAP 항체 (H-9) Alexa Fluor® 680

sc-271134 AF680
200 µg/ml
$357.00

YAP 항체 (H-9) Alexa Fluor® 790

sc-271134 AF790
200 µg/ml
$357.00

YAP (H-9) 중화펩타이드

sc-271134 P
100 µg/0.5 ml
$68.00

why using this antibody staining YAP in MCF-7 is located in cytoplasm

Asked by: likeminem
Thank you for your question. Many proteins localize to more than one location in a cell, so localization often varies depending on the cells. YAP has been shown to translocate to the cytoplasm due to phosphorylation and cell density.
Answered by: Tech Service
Date published: 2019-11-06

which yap antibody from your company that can detect phosphorylated yap?

Asked by: siti
Thank you for your question. We do not have a phospo-YAP antibody that specifically detects only phosphorylated YAP. All the antibodies are capable of detecting phosphorylated YAP as well as non-phosphorylated YAP, depending on the epitope and the phosphorylation site.
Answered by: Tech Service
Date published: 2019-09-12

您好,(sc-271134) YAP Antibody (H-9) 免疫染色的示意图显示在HELA细胞的细胞质,另一支抗体(sc-101199) YAP antibody (63.7) 免疫染色示意图显示,YAP在HELA细胞的细胞核,请问这两只抗体有什么差别?

Asked by: Anonymous
感谢您的提问。请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”。 Thank you for your inquiry. Please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 9
Date published: 2019-05-05

Dear company, Could you please help me to consult the price and inventory of the product YAP Antibody (H-9): sc-271134.Many thanks. Yours, Grace

Asked by: Grace Li
Thank you for your question. Please contact one of our authorized distributors for information about current product availability and shipping lead times.
Answered by: Technical Support
Date published: 2019-04-13

What secondary reagent should I use for IHC if I am working with mice tissue samples?

Asked by: Trav11
For cases like this, we recommend using our mouse monoclonal primary antibodies that are directly conjugated. The YAP Antibody (H-9): sc-271134 is NOT currently available directly conjugated, but we do offer an alternative, YAP Antibody (G-6): sc-376830, that is directly conjugated. Currently, sc-376830 is available conjugated to HRP (sc-376830 HRP), phycoerythrin (sc-376830 PE), fluorescein (sc-376830 FITC), Alexa Fluor® 488 (sc-376830 AF488) or Alexa Fluor® 647 (sc-376830 AF647).
Answered by: Tech Service 8
Date published: 2016-12-22
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Rated 5 out of 5 by from mouse anti-YAP works well for ICCAnti-YAP works well for ICC on murine brown adipocytes.
Date published: 2023-09-22
Rated 5 out of 5 by from Great antibody!It works well in immunofluorescence. 1:100 dilution
Date published: 2021-10-21
Rated 3 out of 5 by from Bright cytosolic staining, limited nuclear staininMDCKs fixed in 4% paraformaldehyde, stained with primary at 1:100 3 hr at 37C. Secondary goat anti-mouse (Dylight 488) overnight at 4C
Date published: 2018-11-27
Rated 4 out of 5 by from The fluorescence is not so nice in Western Blot.This antibody worked in the Western blot, but the fluorescence is not so nice. It seems to recognize only the cytosolic YAP.
Date published: 2018-05-17
Rated 5 out of 5 by from Good qualityI used this antibody for IHC and the result is good.
Date published: 2018-04-25
Rated 5 out of 5 by from Great featuresI used this antibody on nuclear extraction of pancreatic cancer cell line and have good bands.
Date published: 2018-03-14
Rated 3 out of 5 by from YAP 抗体 (H-9) X ChIP实验我们用这个抗体做ChIP实验,但是没有得到阳性结果,有可能该蛋白并不结合我们的目标区域,也可能是抗体问题,目前无法确认。
Date published: 2017-12-11
Rated 5 out of 5 by from Clean band at expected molecular weight (~65kDa)Sample: MDA-MB-231 wcl (30ug) Blocking: 1h in blocking buffer (0.05% tween-20 in TBS with 5% skin milk). Primary antibody: 1:200 in blocking buffer @ 4oC, overnight. Secondary antibody: 1:3000 in blocking buffer. Exposure time: 30 seconds.
Date published: 2017-11-18
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YAP 항체 (H-9) is rated 4.7 out of 5 by 19.
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