Date published: 2025-9-6
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YAP Antibody (G-6) - Western Blotting - Image 393074
YAP1 항체(G-6) HRP: sc-376830 HRP. HeLa 핵 추출물(A) 및 Hep G2(B), AT-3(C) 및 HeLa(D) 전세포 용해물에서 YAP 발현의 직접 웨스턴 블롯 분석.
YAP1 항체(G-6) HRP: sc-376830 HRP. HeLa 핵 추출물(A) 및 Hep G2(B), AT-3(C) 및 HeLa(D) 전세포 용해물에서 YAP 발현의 직접 웨스턴 블롯 분석.

YAP 항체 (G-6): sc-376830

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데이터 시트
  • YAP 항체 G-6 는 마우스 monoclonal IgG1 κ YAP 항체, 80간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 YAP에서 C-terminus에 위치한 206-330 아미노산을 항원으로 사용하였습니다.
  • YAP 항체 (G-6)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 YAP 를 감지하는 데에 추천한다.
  • 항-YAP 항체(G-6)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-376830 X, 200 µg/0.1 ml)이 있습니다.
  • YAP (G-6): sc-376830의 무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP 와 m-IgG1 BP-HRP 는 YAP 항체 (G-6) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은YAP 항체(G-6) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다)

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YAP 항체(G-6)는 마우스, 쥐 및 인간 유래의 YAP 단백질을 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgG1 κ 마우스 모노클로날 YAP 항체(YAP1 항체로도 지정됨)입니다. YAP 항체(G-6)는 비접합 항-YAP 항체 형태뿐만 아니라 아가로스, HRP, PE, FITC 및 여러 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항-YAP 항체 형태로도 사용할 수 있습니다. 예스 관련 단백질은 예스(Yes)의 SH3 도메인에 결합하는 능력으로 인해 원래 알려진 14-3-3 결합 분자입니다. YAP와 14-3-3의 결합은 YAP 14-3-3 결합 모티프에 있는 상동 세린 잔기(Ser 112)의 인산화가 필요합니다. 고도로 보존되고 어디에나 존재하는 14-3-3 단백질은 신호 전달에 관여하는 세포 내 인산화 단백질과 결합하여 분화, 세포 주기 진행 및 세포 사멸을 조절합니다. YAP는 14-3-3 단백질에 의해 조절되는 방식으로 원형질막과 세포 골격에서의 이벤트를 핵에서의 전사 억제와 연결할 수 있습니다. YAP는 전사 인자에 존재하는 PPXY 모티프에 결합하여 전사 보조 인자로 작용하는 PDZ 결합 모티프를 가진 전사 보조 인자인 TAZ의 WW 도메인과 상동성을 공유합니다. YAP는 폐, 태반, 전립선, 난소 및 고환에서 높은 수준으로 발현됩니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

YAP 항체 (G-6)

sc-376830
200 µg/ml
$316.00

YAP (G-6): m-IgG Fc BP-HRP 번들

sc-527550
200 µg Ab; 10 µg BP
$354.00

YAP (G-6): m-IgG1 BP-HRP 번들

sc-532923
200 µg Ab; 20 µg BP
$354.00

YAP 항체 (G-6) X

sc-376830 X
200 µg/0.1 ml
$316.00

YAP 항체 (G-6) AC

sc-376830 AC
500 µg/ml, 25% agarose
$416.00

YAP 항체 (G-6) HRP

sc-376830 HRP
200 µg/ml
$316.00

YAP 항체 (G-6) FITC

sc-376830 FITC
200 µg/ml
$330.00

YAP 항체 (G-6) PE

sc-376830 PE
200 µg/ml
$343.00

YAP 항체 (G-6) Alexa Fluor® 488

sc-376830 AF488
200 µg/ml
$357.00

YAP 항체 (G-6) Alexa Fluor® 546

sc-376830 AF546
200 µg/ml
$357.00

YAP 항체 (G-6) Alexa Fluor® 594

sc-376830 AF594
200 µg/ml
$357.00

YAP 항체 (G-6) Alexa Fluor® 647

sc-376830 AF647
200 µg/ml
$357.00

YAP 항체 (G-6) Alexa Fluor® 680

sc-376830 AF680
200 µg/ml
$357.00

YAP 항체 (G-6) Alexa Fluor® 790

sc-376830 AF790
200 µg/ml
$357.00

Hi, I'm working on IF staining of YAP in fibroblasts, what fixation buffer do you recommend for that with YAP Antibody (G-6): sc-376830? And how about the cell confluence? Will lower or higher confluence influence the YAP location in cells?

Asked by: pan16
Thank you for your question . We recommend using formalin for fixation and cell confluence varies from protein to protein and is determined by empirical data.
Answered by: BlakeJ
Date published: 2023-02-23

what is the certain antibody for rabbit YAP WB test (anti-YAP (1 : 1000; Santa Cruz, USA) in the study <Hydrostatic Pressure Modulates Intervertebral Disc Cell Survival and Extracellular Matrix Homeostasis via Regulating Hippo-YAP/TAZ Pathway>?

Asked by: Markhu
Thank you for your question. We don't know the certain antibody for YAP about this paper. If the author writes both clone number and protein name, then we can know the specific antibody. For YAP, we provide related products, please check the link below. https://www.scbt.com/search?Ntt=YAP1 感谢提问。 这篇文章中，我们并不知道，作者具体使用了我司哪个货号的抗体。 只有当作者同时写明了抗体的克隆号和蛋白名/基因名时，我们才知道具体的货号。 关于YAP，我们公司有如下的相关产品，请查看链接。 https://www.scbt.com/search?Ntt=YAP1
Answered by: Ling Chen
Date published: 2022-07-07

请问这个抗体是可以检测不同亚型的蛋白吗？他们的kd值是否有明显差别？

Asked by: grazylee
Thanks for your question. Anti-YAP1 Antibody (G-6) is recommended for detection of total YAP. Molecular Weight of YAP: 65 kDa. 感谢您的提问。Anti-YAP1 抗体(G-6) 推荐检测总 YAP ，YAP分子量是65kDa。
Answered by: Yanru Tao
Date published: 2022-06-09

Can this antibody be used in huaman BMMSCS samples in WB?

Asked by: AngelWang
Thank you for your question. We have not tested this antibody specifically on Bone Marrow Mesenchymal Stem Cells, so I am unable to specifically say if the YAP protein is expressed in these cells. We recommend you research in the primary literature to see if these BMMSCS cells express the YAP protein.
Answered by: Tech Service
Date published: 2019-12-02

Has anyone tried this anybody for ChIP? Our lab used the predecessor antibody (now discontinued) for ChIP and I need to do it again looking for some other binding sites.

Asked by: Hope34Me
Thank you for your question. We are not currently aware of any data or product citations using this antibody for ChIP research applications. We do offer an alternative antibody, YAP Antibody (63.7): sc-101199, which has been published in ChIP research applications.
Answered by: Technical Support
Date published: 2019-06-16

Is antigen retrieval recommended for this antibody in IHC? If so, are there any guidelines about which antigen retrieval methods are best for the protein?

Asked by: annag16
Thank you for your question. We recommend antigen retrieval for this antibody, sodium citrate with heat or EDTA are the preferred methods. You can find the recommended IHC protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Tech Service
Date published: 2019-01-14

Hello, I'm interested in getting  a FREE 10 µg sample of YAP (G-6): sc-376830. We are working with the mouse intestinal organoids and would like to detect how YAP protein localisation changes during oncoprotein activation. I would appreciate your reply.

Asked by: hui99
Thank you for your question. If you have an interest in testing a free 10 µg sample of sc-376830, please contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21

Can this mouse monoclonal antibody be used to stain mouse samples in IF and IHC?

Asked by: AbPolly
Thank you for your question. Yes, this antibody is recommended for use in IF and IHC with mouse samples. We recommend using a directly conjugated version of the antibody. This YAP antibody, sc-376830, is available conjugated to HRP, PE, FITC, AF488 and AF647.
Answered by: Technical Support 14
Date published: 2017-01-20
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Rated 5 out of 5 by from Good antibody for WBThe antibody showed great reactivity in WB using several human cell lines.
Date published: 2024-08-23
Rated 4 out of 5 by from Good.YAP antibody detected reasonably in human stromal cells by WB, dilution 1:500.
Date published: 2022-04-18
Rated 5 out of 5 by from Great antibody!It works well in immunofluorescence. 1:100 dilution
Date published: 2021-10-21
Rated 1 out of 5 by from the batch A2220 does not work at all!the batch A2220 does not work at all! Gives a band at the wrong size lower than 60Kda, and a strong laddering above. Another batch of the same ref did work with my samples.
Date published: 2020-12-10
Rated 5 out of 5 by from Good at WB/IFNice antibody for Immunofluorescence and western blotting!
Date published: 2020-11-30
Rated 4 out of 5 by from 功能很出色我一个月前购买此产品，并为此感到高兴，之后有需要的话会再购买，主要是抗体浓度需要提高到很高希望能在这方面加以改善，谢谢
Date published: 2020-10-22
Rated 5 out of 5 by from Nice and single band (w.b.)Produced a single band at the expected molecular weight (~65kDa). Sample: MDA-MB-231 wcl (30ug). Blocking: 1h in blocking buffer (0.05% tween-20 in TBS with 5% skin milk). Primary antibody: 1:200 in blocking buffer, overnight @ 4oC. Secondary antibody: 1:3000 in blocking buffer. Exposure time: 30 seconds* *further dilution of the primary antibody (1:400-1:500 ?) may yield similar results at the expense of a prolonged exposure time (2-4 minutes).
Date published: 2017-11-18
Rated 5 out of 5 by from Good antibody!I used this antibody for WB. I detected a band at 65 kDa. I'm satisfied with the antibody.
Date published: 2017-11-06
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YAP 항체 (G-6) is rated 4.7 out of 5 by 30.
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