VCP 항체 (5) WB, IP, IF와 IHC(P)으로 mouse, rat, human와 porcine origin의 VCP을 검출할것을 권장합니다.

VCP 항체 (5): sc-57492

VCP 항체 (5) is rated 4.8 out of 5 by 10.
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Datasheets

    • 안티-VCP 항체 5 는 마우스 monoclonal IgG2b (kappa light chain) VCP 항체 이며 200 µg/ml으로 제공합니다.
    • human origin의 VCP에서 내부에 위치한 792-806 아미노산을 항원으로 사용하였습니다.
    • 안티-VCP 항체 (5)는 WB, IP, IF and IHC(P)으로 mouse, rat, human and porcine유래의 VCP 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-VCP 항체 (5)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • 강력추천하는 conventional secondary anti-mouse IgG reagents의 대체제품 보기m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP).
    • VCP (5): sc-57492무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
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I am using VCP (5): sc-57492 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Perfect for ICC Funciona excelentemente para ICC de fibroblastos primarios usandolo hasta 1:500 en PBST (0.1% Triton) + 10% Serum 1h RT u O/N 4ºC.
Date published: 2020-02-19
Rated 5 out of 5 by from Works for both IF and Western blot Brought this antibody 6 months ago. It works for both western blot and IF staining very well.
Date published: 2019-09-05
Rated 5 out of 5 by from Best VCP Antibody ever! Very clean and very specific! We use it to detect endogenous protein and it works great at 1:1000 loading very little lysate (10ug or less)
Date published: 2018-01-31
Rated 5 out of 5 by from Works very well on mouse liver WB Works very well on mouse liver lysates western blotting. very clean bands.
Date published: 2017-10-10
Rated 5 out of 5 by from Great for WB! Consistent, clean and strong bands seen across multiple whole cell lysate samples in Western Blot.
Date published: 2017-01-23
Rated 5 out of 5 by from good Abs this Abs was good for IP both on human and mouse cells
Date published: 2016-11-11
Rated 5 out of 5 by from Excellent Antibody I used this antibody on my Westerns and it worked very well.
Date published: 2016-10-31
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmic Produced excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human spleen tissue. -SCBT QC
Date published: 2015-05-13
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