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V-ATPase D1 항체 (34-Z): sc-81887

4.0(5)
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데이터 시트
  • V-ATPase D1 항체(34-Z) 는 마우스 monoclonal IgG1κ, 7간행물에 인용, 이며 100 µg/ml으로 제공합니다.
  • human origin의 V-ATPase D1의 전체인 238-309 아미노산을 항원으로 사용하였습니다.
  • WB, IP, IF, IHC(P) 와ELISA으로 human origin의 V-ATPase D1을 검출할것을 권장합니다.
  • 현재 V-ATPase D1 항체(34-Z)에 대해 선호하는 2차 검출 시약의 식별을 아직 완료하지 못했습니다. 이 작업은 진행 중입니다.

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Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for the acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and an integral V0 domain, which is responsible for proton translocation, comprise the V-ATPase complex. Nine subunits (A-H) make up the V1 domain and five subunits (A, D, C, C' and C") make up the V0 domain. V-ATPase D1 (ATPase, H+ transporting, lysosomal, V0 subunit D1), also known as ATP6V0D1, P39, VATX, VMA6, ATP6D or VPATPD, is the D subunit of the V0 domain. Expressed ubiquitously, V-ATPase D1 acts in concert with other V0 subunits to catalytically acidify a variety of intracellular compartments, thereby synthesizing ATP to be used for vacuolar transport.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

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V-ATPase D1 항체 (34-Z)

sc-81887
100 µg/ml
$333.00

I detected only 100kDa band and weak band under 37kDa in HUVECs using this antibody. That's why I am asking about the transfected data in the data sheets.  Can this antibody detect other component of V-ATP ase?

Asked by: Maxi
Thank you for your question. Please contact our Technical Service Department or your local distributor for assistance in troubleshooting this antibody for Western blotting.
Answered by: Tech Service
Date published: 2019-11-06

The molecular weight of V-ATPase D1 is 40kDa and the WB data of Hela in the data sheet is 40kDa. But WB of human V-ATPase D1 transfected 293T cells in the data sheet is around 100kDa. Which is the true molecular weight?

Asked by: Maxi
Thank you for your question. Thank you for bringing this discrepancy to our attention. The observed molecular weight in the transfected lysate is much higher than expected. We will be reviewing this data for accuracy and will remove or replace it if necessary.
Answered by: Technical Support
Date published: 2019-11-06

Can sc-81887: V-ATPase D1 (34-Z) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Professor Griffin
Thank you for your question. Yes, sc-81887: V-ATPase D1 (34-Z) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
  • y_2025, m_8, d_28, h_5CST
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Rated 4 out of 5 by from Good specificity observed in WB in HeLa wholeGood specificity observed in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-04-26
Rated 4 out of 5 by from Produced robust immunoperoxidase cytoplasmicProduced robust immunoperoxidase cytoplasmic staining in formalin-fixed, paraffin-embedded human stomach tissue. -SCBT QC
Date published: 2015-03-31
Rated 4 out of 5 by from Published data for WB in HeLa cellsPublished data for WB in HeLa cells. -SCBT Publication Review
Date published: 2015-03-28
Rated 4 out of 5 by from Good antibody for WB in human VGood antibody for WB in human V-ATPase D1 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-03-13
Rated 4 out of 5 by from Published data for WB in epithelial tissuesPublished data for WB in epithelial tissues from human breast cancer tissue. -SCBT Publication Review
Date published: 2015-01-12
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V-ATPase D1 항체 (34-Z) is rated 4.0 out of 5 by 5.
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