Date published: 2025-9-6

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TNF alpha 항체 (C-4): sc-133192

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데이터 시트
  • TNF alpha 항체(C-4) 는 마우스 monoclonal IgG2aκ, 54간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 TNFα에서 내부에 위치한 77-233 아미노산을 항원으로 사용하였습니다.
  • TNF alpha 항체 (C-4)는 WB, IP, IF and ELISA으로 human유래의 TNFα 를 감지하는 데에 추천한다 .
  • 항-TNF alpha 항체(C-4)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • TNF alpha (C-4): sc-133192무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP, 2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP는 TNF alpha 항체(C-4) WB 애플리케이션용입니다. 이 시약은 이제 TNF alpha 항체(C-4)와 함께 번들로 제공됩니다(아래 주문 정보 참조).

빠른 링크

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TNFα 항체(C-4)는 IgG2a κ 마우스 단일 클론 TNFα 항체(TNFα 항체 또는 TNF 알파 항체라고도 함)로 WB, IP, IF 및 ELISA로 인간 유래의 TNFα를 검출합니다. TNFα 항체(C-4)는 비접합 항-TNFα 항체 형태뿐만 아니라 아가로스, HRP, PE, FITC 및 여러 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항-TNFα 항체 형태로도 사용할 수 있습니다. 림프독소라고도 알려진 종양 괴사 인자 β(TNFβ)는 형질 전환성 사이토카인입니다. 카섹틴으로도 알려진 TNFα는 동일한 수용체에 결합하는 더 작은 사이토카인으로, TNFβ와 유사한 광범위한 효과를 생성합니다. TNFβ와 TNFα는 30%의 아미노산 상동성을 공유하며 생물학적 활성도 비슷합니다. TNFβ는 CD4+ T 헬퍼 세포 1형 림프구, CD8+ 림프구 및 특정 B 림포모구 세포주를 포함한 활성화된 림프구에서 생성됩니다. TNFα는 림프구, 호중구 및 대식세포를 포함한 여러 가지 세포 유형에서 생성됩니다. TNFα와 TNFβ는 많은 면역 및 염증 기능을 조절하는 동시에 종양 성장을 억제하는 능력을 가지고 있습니다. 표적 종양 세포가 사멸하려면 TNF 수용체 1과 2를 발현해야 하며, p55 수용체가 세포 독성 반응을 매개합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

TNF alpha 항체 (C-4)

sc-133192
200 µg/ml
$316.00

TNF alpha (C-4): m-IgG Fc BP-HRP 번들

sc-528765
200 µg Ab; 10 µg BP
$354.00

TNF alpha (C-4): m-IgGκ BP-HRP 번들

sc-521270
200 µg Ab, 40 µg BP
$354.00

TNF alpha (C-4): m-IgG2a BP-HRP 번들

sc-547163
200 µg Ab; 10 µg BP
$354.00

TNF alpha 항체 (C-4) AC

sc-133192 AC
500 µg/ml, 25% agarose
$416.00

TNF alpha 항체 (C-4) HRP

sc-133192 HRP
200 µg/ml
$316.00

TNF alpha 항체 (C-4) FITC

sc-133192 FITC
200 µg/ml
$330.00

TNF alpha 항체 (C-4) PE

sc-133192 PE
200 µg/ml
$343.00

TNF alpha 항체 (C-4) Alexa Fluor® 488

sc-133192 AF488
200 µg/ml
$357.00

TNF alpha 항체 (C-4) Alexa Fluor® 546

sc-133192 AF546
200 µg/ml
$357.00

TNF alpha 항체 (C-4) Alexa Fluor® 594

sc-133192 AF594
200 µg/ml
$357.00

TNF alpha 항체 (C-4) Alexa Fluor® 647

sc-133192 AF647
200 µg/ml
$357.00

TNF alpha 항체 (C-4) Alexa Fluor® 680

sc-133192 AF680
200 µg/ml
$357.00

TNF alpha 항체 (C-4) Alexa Fluor® 790

sc-133192 AF790
200 µg/ml
$357.00

what's the difference between sc-133192 and sc-52746? Are they all applicable for IF staining of rat liver tissue?

Asked by: Chuman
Thank you for your question. sc-133192 is raised against amino acids 77-233 of TNFα of human origin. sc-133192 is recommended for detection of TNFα of human origin by WB, IP, IF and ELISA. sc-52746 is raised against purified full length native TNFα of human origin. sc-52746 is recommended for detection of natural and recombinant TNFα of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA; also reactive with additional species, including and Guinea pig, Chimpanzee, Cynomolgus and Rhesus monkey; non cross-reactive with TNFβ or lymphotoxin.
Answered by: Ling Chen
Date published: 2025-04-21

Is a blocking peptide for this product available?

Asked by: dragonfly
Thank you for your question. This mouse monoclonal antibody, TNFα (C-4): sc-133192 is not raised against a peptide so no blocking peptide is available. The antibody is raised against amino acids 77-233 of TNFα of human origin.
Answered by: Technical Support
Date published: 2025-01-15

What is the recommended starting dilution for Western blot for TNFα (C-4): sc-133192?

Asked by: jenni
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-133192.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 8
Date published: 2025-01-25
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Rated 5 out of 5 by from good for WBI can detect tnfa by WB. I used macrophage (RAW264.7) and macrophage CM.
Date published: 2018-07-13
Rated 2 out of 5 by from Didn't detect any TNF in my blotsI am not sure if the antibody worked or not because I was not able to detect any TNF in my western blots and did not run a positive control.
Date published: 2018-03-13
Rated 4 out of 5 by from sc-133192We used it to detect brain samples. A clear band at the correct size. There are two more prominent bands at other sizes, too
Date published: 2017-10-03
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 4 out of 5 by from sc-133192we use it on Caco cells using WB,. Work well.
Date published: 2017-08-21
Rated 3 out of 5 by from NOT VERY GOODTNF-A is not good to IHC,but other use not to do it .
Date published: 2017-07-21
Rated 3 out of 5 by from band is fuzzythe antibody samples arrived soon, but the protein band is fuzzy
Date published: 2017-07-18
Rated 3 out of 5 by from applicate it into IHCwe've applicated this antibody into the experiment of IHC , but the result is not we want because we got the positive results but in the published papers they are the negative results
Date published: 2017-05-31
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TNF alpha 항체 (C-4) is rated 4.1 out of 5 by 17.
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