TMPRSS2 항체 (H-4): sc-515727

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- 안티-TMPRSS2 항체 H-4 는 마우스 monoclonal IgG₁ κ TMPRSS2 항체, 51간행물에 인용, 이며 200 µg/ml으로 제공합니다.
- 아미노 산 296-345 mapping within an internal region of TMPRSS2 of human 의하고 기원한다
- 안티-TMPRSS2 항체 (H-4)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 TMPRSS2 catalytic chain 를 감지하는 데에 추천한다.
- IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-TMPRSS2 항체 (H-4)를 제공한다.
- WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 or Alexa Fluor^® 647결합제품도 있습니다.
- WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR^®Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor^® 680 or Alexa Fluor^® 790 결합제품도 있습니다.
- TMPRSS2 (H-4): sc-515727의 무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
- m-IgG Fc BP-HRP, m-IgG₁ BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TMPRSS2 Antibody (H-4) for WB and IHC(P) applications. These reagents are now offered in bundles with TMPRSS2 Antibody (H-4) (see ordering information below).

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TMPRSS4를 위한 TMPRSS 항체
Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional

Anti-TMPRSS2 Antibody (H-4) - Western Blotting - Image 396555

Anti-TMPRSS2 Antibody (H-4) - Western Blotting - Image 379481

Anti-TMPRSS2 Antibody (H-4) - Western Blotting - Image 384568

Anti-TMPRSS2 Antibody (H-4) - Western Blotting - Image 396554

Anti-TMPRSS2 Antibody (H-4) - Western Blotting - Image 396556

Anti-TMPRSS2 Antibody (H-4) - Western Blotting - Image 396557

Anti-TMPRSS2 Antibody (H-4) - Western Blotting - Image 396699

Anti-TMPRSS2 Antibody (H-4) - Western Blotting - Image 396558

Anti-TMPRSS2 Antibody (H-4) - Western Blotting - Image 359938

Anti-TMPRSS2 Antibody (H-4) - Western Blotting - Image 396487

Anti-TMPRSS2 Antibody (H-4) - Western Blotting - Image 386137

Anti-TMPRSS2 Antibody (H-4) - Western Blotting - Image 379477

Anti-TMPRSS2 Antibody (H-4) - Western Blotting - Image 399340

이미지나 확대버튼을 클릭하면 확대이미지를 열람할수있습니다.

TMPRSS2 Antibody (H-4) is a high quality monoclonal TMPRSS2 antibody (also designated PRSS10 antibody or Transmembrane protease serine 2 antibody) suitable for the detection of the TMPRSS2 protein of mouse, rat and human origin. TMPRSS2 Antibody (H-4) is available as both the non-conjugated anti-TMPRSS2 antibody form, as well as multiple conjugated forms of anti-TMPRSS2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Extracellular proteases mediate the digestion of neighboring extracellular matrix components in initial tumor growth, allow desquamation of tumor cells into the surrounding environment, provide the basis for invasion of basement membranes in targeted metastatic organs and are required for release and activation of many growth and angiogenic factors. The TMPRSS2 gene encodes a 492 amino acid multimeric serine protease, which is mainly expressed in the mouse prostate and kidney, and is also expressed in the human small intestine, prostate, colon, stomach and salivary gland. TMPRSS2 contains several domains, including a serine protease domain of the S1 family, a scavenger receptor cysteine-rich domain of group A, an LDL receptor class A domain and a transmembrane domain. TMPRSS2 is expressed as a full length form and a cleaved protease domain and its expression is increased by androgenic hormones. TMPRSS2 is also expressed in prostate carcinoma, suggesting that it may play a role in prostate carcinogenesis.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number
Human	TMPRSS2	7113	21q22.3	XM_005261043, XM_011529731, XM_011529732, XM_011529733, NM_001135099, NM_005656	O15393	602060
Mouse	Tmprss2	50528	16 C4	XM_006523064, XM_006523065, NM_015775	Q9JIQ8	N/A

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Dear technical support, Has this antibody been validated using KO or silencing? We've tested 4 different anti TMPRSS2 antibodies so far they all give a band at the right siez but 1) even in lysates from cells known not to express TMPRSS2 2) even in KO!

Asked by: CGJvirus

Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.

Answered by: Technical Support

Date published: 2021-07-22

Dear technical support team, I would like to use your TMPRSS2 (H-4) -PE antibody for flow cytometric analysis. Was this antibody validated for flow cytometry and does it require cell permeabilization?

Asked by: ealeksa2

Thank you for your question. TMPRSS2 Antibody (H-4): sc-515727 was raised against amino acids 296-345 mapping within an internal region of TMPRSS2 of human origin, which correspond to the extracellular domain of the protein. The antibody has not been validated for flow cytometry.

Answered by: Technical Support Europe

Date published: 2020-10-08

Dear Technical Support Team, we hope to use the TMPRSS2 Antikörper (H-4): sc-515727 PE in flow cytometry. Which dilution/ µg of antibody do you recommend per 1 Mio cells?

Asked by: LiMS

Thank you for your question. We recommend using 1 µg antibody (5 µl) per 1 million cells.

Answered by: Tech Support Europe

Date published: 2020-08-04

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with TMPRSS2 (H-4): sc-515727?

Asked by: Cweed

We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety

Answered by: Technical Support

Date published: 2017-02-27

Rated 4 out of 5 by Anonymous from Good antibody I used this antibody fo immunofluorescence in Calu-3 and Hek293 cells. I actually visualised the antibody only in Calu-3 cells, as expected. Good staining.

Date published: 2021-05-04

Rated 5 out of 5 by Anonymous from Works for immunofluorescence Used it to visualize ectopically expressed TMPRSS2 in HEK293 cells by IF. Gave good signal with apparent cell surface expression, as expected. This was a courtesy replacement for sc-101847, which gave no signal at all.

Date published: 2020-12-30

Rated 4 out of 5 by mousetaro from Working in WB human TMPRSS2 expressed in mice was successfully detected by WB.

Date published: 2020-11-21

Rated 4 out of 5 by sumolikehero from Good product and works well. I bought a month ago and it works for detecting TMPRSS2 proteins.

Date published: 2020-07-14

Rated 5 out of 5 by Mao123 from Excellent in western blotting Dilution 1.1000 Western Blotting cell lysate of MDA, C2C12, HepG2 Very stong and specific signals. Detection changes according the cell type (different glycosilate forms)m

Date published: 2020-05-18

Rated 1 out of 5 by Alexandr from Kein spezifisches Signal Bei der Expression in Oozyten haben wir kein spezifisches Signal in Ganzzelllysaten detektiert. Starkes Signal bei etwa 55 kDa ist unspezifisch und auch in Kontrollgruppe vorhanden.

Date published: 2020-05-18

Rated 5 out of 5 by 翎001 from effective i recieved this antibody 2 momths ago for free trial, and i'm happy to got a band in the right place (1:100 dilution), band is clear

Date published: 2017-08-15

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TMPRSS2 항체 (H-4): sc-515727

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Dear technical support, Has this antibody been validated using KO or silencing? We've tested 4 different anti TMPRSS2 antibodies so far they all give a band at the right siez but 1) even in lysates from cells known not to express TMPRSS2 2) even in KO!

Dear technical support team, I would like to use your TMPRSS2 (H-4) -PE antibody for flow cytometric analysis. Was this antibody validated for flow cytometry and does it require cell permeabilization?

Dear Technical Support Team, we hope to use the TMPRSS2 Antikörper (H-4): sc-515727 PE in flow cytometry. Which dilution/ µg of antibody do you recommend per 1 Mio cells?

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with TMPRSS2 (H-4): sc-515727?

TMPRSS2 항체 (H-4): sc-515727

빠른 링크

더보기

Dear technical support, Has this antibody been validated using KO or silencing? We've tested 4 different anti TMPRSS2 antibodies so far they all give a band at the right siez but 1) even in lysates from cells known not to express TMPRSS2 2) even in KO!

Dear technical support team, I would like to use your TMPRSS2 (H-4) -PE antibody for flow cytometric analysis. Was this antibody validated for flow cytometry and does it require cell permeabilization?

Dear Technical Support Team, we hope to use the TMPRSS2 Antik&ouml;rper (H-4): sc-515727 PE in flow cytometry. Which dilution/ &micro;g of antibody do you recommend per 1 Mio cells?

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with TMPRSS2 (H-4): sc-515727?

Dear Technical Support Team, we hope to use the TMPRSS2 Antikörper (H-4): sc-515727 PE in flow cytometry. Which dilution/ µg of antibody do you recommend per 1 Mio cells?