TMPRSS2 항체 (H-4) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 TMPRSS2 catalytic chain을 검출할것을 권장합니다.

TMPRSS2 항체 (H-4): sc-515727

TMPRSS2 항체 (H-4) is rated 4.0 out of 5 by 7.
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데이터 시트

    • 안티-TMPRSS2 항체 H-4 는 마우스 monoclonal IgG1 κ TMPRSS2 항체, 51간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • 아미노 산 296-345 mapping within an internal region of TMPRSS2 of human 의하고 기원한다
    • 안티-TMPRSS2 항체 (H-4)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 TMPRSS2 catalytic chain 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-TMPRSS2 항체 (H-4)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • TMPRSS2 (H-4): sc-515727무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TMPRSS2 Antibody (H-4) for WB and IHC(P) applications. These reagents are now offered in bundles with TMPRSS2 Antibody (H-4) (see ordering information below).
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Dear technical support, Has this antibody been validated using KO or silencing? We've tested 4 different anti TMPRSS2 antibodies so far they all give a band at the right siez but 1) even in lysates from cells known not to express TMPRSS2 2) even in KO!

Asked by: CGJvirus
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Dear technical support team, I would like to use your TMPRSS2 (H-4) -PE antibody for flow cytometric analysis. Was this antibody validated for flow cytometry and does it require cell permeabilization?

Asked by: ealeksa2
Thank you for your question. TMPRSS2 Antibody (H-4): sc-515727 was raised against amino acids 296-345 mapping within an internal region of TMPRSS2 of human origin, which correspond to the extracellular domain of the protein. The antibody has not been validated for flow cytometry.
Answered by: Technical Support Europe
Date published: 2020-10-08

Dear Technical Support Team, we hope to use the TMPRSS2 Antikörper (H-4): sc-515727 PE in flow cytometry. Which dilution/ µg of antibody do you recommend per 1 Mio cells?

Asked by: LiMS
Thank you for your question. We recommend using 1 µg antibody (5 µl) per 1 million cells.
Answered by: Tech Support Europe
Date published: 2020-08-04

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with TMPRSS2 (H-4): sc-515727?

Asked by: Cweed
We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Good antibody I used this antibody fo immunofluorescence in Calu-3 and Hek293 cells. I actually visualised the antibody only in Calu-3 cells, as expected. Good staining.
Date published: 2021-05-04
Rated 5 out of 5 by from Works for immunofluorescence Used it to visualize ectopically expressed TMPRSS2 in HEK293 cells by IF. Gave good signal with apparent cell surface expression, as expected. This was a courtesy replacement for sc-101847, which gave no signal at all.
Date published: 2020-12-30
Rated 4 out of 5 by from Working in WB human TMPRSS2 expressed in mice was successfully detected by WB.
Date published: 2020-11-21
Rated 4 out of 5 by from Good product and works well. I bought a month ago and it works for detecting TMPRSS2 proteins.
Date published: 2020-07-14
Rated 5 out of 5 by from Excellent in western blotting Dilution 1.1000 Western Blotting cell lysate of MDA, C2C12, HepG2 Very stong and specific signals. Detection changes according the cell type (different glycosilate forms)m
Date published: 2020-05-18
Rated 1 out of 5 by from Kein spezifisches Signal Bei der Expression in Oozyten haben wir kein spezifisches Signal in Ganzzelllysaten detektiert. Starkes Signal bei etwa 55 kDa ist unspezifisch und auch in Kontrollgruppe vorhanden.
Date published: 2020-05-18
Rated 5 out of 5 by from effective i recieved this antibody 2 momths ago for free trial, and i'm happy to got a band in the right place (1:100 dilution), band is clear
Date published: 2017-08-15
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