TIMP-2 항체 (H-4) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 TIMP-2을 검출할것을 권장합니다.

TIMP-2 항체 (H-4): sc-271932

TIMP-2 항체 (H-4) is rated 5.0 out of 5 by 2.
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데이터 시트

    • TIMP-2 항체(H-4) 는 마우스 monoclonal IgG2aκ, 1간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 TIMP-2에서 내부에 위치한 81-220 아미노산을 항원으로 사용하였습니다.
    • WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 TIMP-2을 검출할것을 권장합니다.
    • See TIMP-2 (3A4): sc-21735 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TIMP-2 Antibody (H-4) for WB and IHC(P) applications. These reagents are now offered in bundles with TIMP-2 Antibody (H-4) (see ordering information below).
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SCBT QC - does this antibody give a nice clean single band on WB? The blot showed in your picture is cut off above 34K do you detect bands higher than 34K?  I would like to use the antibody for IHC.... looking for a nice, really clean Ab

Asked by: Swati Naphade
Thank you for your question. The Western blot data that you are referring to is not representative of this antibody’s detection of endogenous TIMP-2 as it is against a recombinant protein. We do have very good IHC(P) data with this antibody and recommend it for this application. Alternatively, perhaps you would like to consider a more well established antibody with numerous citations for IHC, as well as Western blot and IHC/IF data, TIMP-2 Antibody (3A4): sc-21735 .
Answered by: Technical Support
Date published: 2018-12-11

Can TIMP-2 (H-4): sc-271932 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, TIMP-2 (H-4): sc-271932 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmic Exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human adrenal gland tissue. -SCBT QC
Date published: 2015-02-08
Rated 5 out of 5 by from Satisfactory Western Blot data of human recombinant Satisfactory Western Blot data of human recombinant TIMP-2. -SCBT QC
Date published: 2013-06-11
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