SREBP-2 항체 (C-6) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 SREBP-2을 검출할것을 권장합니다.

SREBP-2 항체 (C-6): sc-271615

SREBP-2 항체 (C-6) is rated 5.0 out of 5 by 2.
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데이터 시트

    • SREBP-2 항체(C-6) 는 마우스 monoclonal IgG2aκ, 6간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 SREBP-2에서 내부에 위치한 812-975 아미노산을 항원으로 사용하였습니다.
    • WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 SREBP-2을 검출할것을 권장합니다.
    • See SREBP-2 (1C6): sc-13552 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for SREBP-2 Antibody (C-6) for WB and IHC(P) applications. This reagent is now offered in a bundle with SREBP-2 Antibody (C-6) (see ordering information below).
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Why do your WBlott samples (Data sh.) as well as the above citations (Liu et al) show a 55/68 kDa mark if the antibody was performed to the C-terminal part of the protein ( not present in the mature form of SREBP) ??? Isn´t that inespecific??

Asked by: tesl
Thank you for your question. There are known cleavage and degradation products for SERBP-2 that run as low as 55kDa, so it's possible to see signal for this protein ranging from that size and up to mature form around 68-70kDa and pre-cursor around 125kDa. It is very dependent on your samples and the status of the protein in your samples.
Answered by: Tech Service
Date published: 2020-01-16

I am using SREBP-2 (C-6): sc-271615 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Beautiful immunoperoxidase cytoplasmic staining Beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human oral mucosa tissue. -SCBT QC
Date published: 2015-03-26
Rated 5 out of 5 by from Great antibody for Western Blot Great antibody for Western Blot, nice signal on LNCaP whole cell lysate. -SCBT QC
Date published: 2015-02-02
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