SREBP-2 항체 (A-12) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 SREBP-2을 검출할것을 권장합니다.

SREBP-2 항체 (A-12): sc-271616

SREBP-2 항체 (A-12) is rated 4.3 out of 5 by 9.
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데이터 시트

    • SREBP-2 항체(A-12) 는 마우스 monoclonal IgG2aκ, 8간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 SREBP-2에서 내부에 위치한 812-975 아미노산을 항원으로 사용하였습니다.
    • 안티-SREBP-2 항체 (A-12)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 SREBP-2 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-SREBP-2 항체 (A-12)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • SREBP-2 (A-12): sc-271616무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for SREBP-2 Antibody (A-12). This work is in progress.
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Does this antibody recognize also the processed (activated) form of SREBP?

Asked by: LaMa
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

I am using SREBP-2 (A-12): sc-271616 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from no result I used a free sample for SREBP2 detection in WB of protein extracts from H9c2 cells. Unfortunately I did not get any results although I have tested various dilutions of the antibody (1: 1000, 1: 500, 1: 250).
Date published: 2019-12-13
Rated 5 out of 5 by from expected results SREBP-2 expression in the rat kidney of type I diabetic, and the results of the antibodies were consistent with the expected results. It was recommended that the dilution should be controlled from 1:800 to 1:1000.
Date published: 2017-09-16
Rated 5 out of 5 by from very sensitive This SREBP-2 antibody is very sensitive. The result gives no background with a very strong signal.I suggest use 1:800 dilution and incubtated at 4℃ overnight instead of RT incubated.
Date published: 2017-09-09
Rated 5 out of 5 by from recommend use Strong and specific, this clone is a great reliable choice for IHC and WB analysis.
Date published: 2017-05-13
Rated 5 out of 5 by from IHC staining IHC staining result is strong for mouse liver tissue slide sections, 1:300 been diluted.
Date published: 2017-02-12
Rated 5 out of 5 by from Well-rounded antibody for SREBP-2 Reliable antibody that gives great staining in IHC on paraffin sections and strong solid bands in Western blot. Excellent choice!
Date published: 2017-02-01
Rated 4 out of 5 by from A large intense band observed by Western A large intense band observed by Western Blot using MOLT-4 whole cell lysate. -SCBT QC
Date published: 2015-05-17
Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmic Exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human oral mucosa tissue. -SCBT QC
Date published: 2015-05-01
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