Sm B/B'/N 항체 (A-5) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 Sm B, Sm B′ and Sm N을 검출할것을 권장합니다.

Sm B/B'/N 항체 (A-5): sc-374009

Sm B/B'/N 항체 (A-5) is rated 4.7 out of 5 by 3.
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데이터 시트

    • Sm B/B'/N 항체(A-5) 는 마우스 monoclonal IgG2a(kappa light chain) 이며 200 µg/ml으로 제공합니다.
    • raised against amino acids 1-240 representing full length SmB of human origin
    • 안티-Sm B/B'/N 항체 (A-5)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 Sm B, Sm B′ and Sm N 를 감지하는 데에 추천한다.; 이외에, equine and canine등 species와 반응할수 있습니다
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Sm B/B'/N 항체 (A-5)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Sm B/B'/N (A-5): sc-374009무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.

Can Sm B/B'/N (A-5): sc-374009 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, Sm B/B'/N (A-5): sc-374009 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from works on immunocytochemistry It works well on immunocytochemistry on HEK293 at 1:100.
Date published: 2017-12-22
Rated 5 out of 5 by from Superb immunoperoxidase nuclear staining Superb immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human brain tissue. -SCBT QC
Date published: 2015-05-20
Rated 5 out of 5 by from WB: strong signal in 293T whole cell lysate WB: strong signal in 293T whole cell lysate and very robust band in KNRK nuclear extract. -SCBT QC
Date published: 2015-05-17
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