Septin 3 항체 (G-6) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 Septin 3을 검출할것을 권장합니다.

Septin 3 항체 (G-6): sc-74431

Septin 3 항체 (G-6) is rated 4.7 out of 5 by 3.
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데이터 시트

    • 안티-Septin 3 항체 G-6 는 마우스 monoclonal IgG2b (kappa light chain) Septin 3 항체 이며 200 µg/ml으로 제공합니다.
    • human origin의 Septin 3에서 내부에 위치한 1-50 아미노산을 항원으로 사용하였습니다.
    • 안티-Septin 3 항체 (G-6)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 Septin 3 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Septin 3 항체 (G-6)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Septin 3 (G-6): sc-74431무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Septin 3 Antibody (G-6) for WB and IHC(P) applications. These reagents are now offered in bundles with Septin 3 Antibody (G-6) (see ordering information below).
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Can Septin 3 (G-6): sc-74431 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes, Septin 3 (G-6): sc-74431 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Produced exceptional immunofluorescence membrane Produced exceptional immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-08
Rated 4 out of 5 by from Good band detected in WB using Jurkat whole Good band detected in WB using Jurkat whole cell lysate. -SCBT QC
Date published: 2015-01-28
Rated 5 out of 5 by from Crisp Crisp, clean band seen in WB using IMR-32 whole cell lysate. -SCBT QC
Date published: 2015-01-03
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