Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SDHB Double Nickase Plasmid (h): sc-401529-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SDHB Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SDHB Double Nickase Plasmid (h) and SDHB Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SDHB. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SDHB Antibody (G-10): sc-271548
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SDHB Double Nickase Plasmid (h) | sc-401529-NIC | 20 µg | $410.00 |
SDHB encodes the iron–sulfur subunit of succinate dehydrogenase (mitochondrial complex II), which couples succinate oxidation in the tricarboxylic acid cycle to electron transfer to ubiquinone in the respiratory chain. Through this dual role, SDHB helps regulate mitochondrial energy metabolism, redox homeostasis, and flux through anaplerotic and biosynthetic pathways. Disruption of SDHB can drive succinate accumulation and downstream signaling changes that impact hypoxia-inducible factor pathways and epigenetic regulation via α-ketoglutarate–dependent dioxygenases. Genetic and functional alterations of SDHB are associated with mitochondrial dysfunction and are widely studied in the context of metabolic reprogramming and tumor susceptibility syndromes.
SDHB Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SDHB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SDHB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SDHB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SDHB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.