rRNA Antibody (Y10b): sc-33678
- rRNA Antibody (Y10b) is a mouse monoclonal IgG2a κ rRNA antibody, cited in 15 publications, provided at 200 µg/ml
- derived from unimmunized autoimmune NZB/NZW mice
- rRNA Antibody (Y10b) is recommended for detection of rRNA of mouse, rat and human origin by IP, IF and IHC(P)
- Anti-rRNA Antibody (Y10b) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for rRNA Antibody (Y10b) for IHC(P) applications. This reagent is now offered in a bundle with rRNA Antibody (Y10b) (see ordering information below).
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SEE ALSO...
rRNA Antibody (Y10b) is a mouse monoclonal IgG2a kappa light chain antibody that detects the rRNA protein of mouse, rat, and human origin by immunoprecipitation (IP) and immunofluorescence (IF). anti-rRNA antibody (Y10b) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ribosomal RNA (rRNA) is a vital component of ribosomes, where rRNA plays a crucial role in the assembly of amino acids into polypeptides, ultimately leading to protein synthesis. The structure of rRNA is particularly important as rRNA forms the core of ribosomal architecture, providing a scaffold for ribosomal proteins to assemble. Eukaryotic ribosomes are composed of four distinct rRNA molecules: 5S, 5.8S, 18S, and 28S, each synthesized in different cellular compartments, with 5.8S, 18S, and 28S being produced in the nucleolus and 5S in the nucleus. These rRNA molecules not only contribute to the structural integrity of the ribosome but also facilitate critical functions such as correctly positioning messenger RNA and peptidyl transferase activity, which is essential for peptide bond formation during translation. The abundance of rRNA, constituting approximately 80% of the total RNA in a typical eukaryotic cell, underscores rRNA′s fundamental role in cellular biology and the significance of rRNA antibody (Y10b) in studying ribosomal function and protein synthesis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
rRNA Antibody (Y10b) References:
- Ribosomal RNA expression in the developing rat olfactory bulb. | Fiske, BK. and Brunjes, PC. 1999. Brain Res Dev Brain Res. 113: 55-60. PMID: 10064874
- Dynamic visualization of local protein synthesis in hippocampal neurons. | Aakalu, G., et al. 2001. Neuron. 30: 489-502. PMID: 11395009
- Ribosomes in the squid giant axon. | Bleher, R. and Martin, R. 2001. Neuroscience. 107: 527-34. PMID: 11719007
- Group I and II metabotropic glutamate receptors are necessary for the activity-dependent regulation of ribosomes in chick auditory neurons. | Nicholas, AH. and Hyson, RL. 2004. Brain Res. 1014: 110-9. PMID: 15212997
- Measurement of dendritic mRNA transport using ribosomal markers. | Kim, HK., et al. 2005. Biochem Biophys Res Commun. 328: 895-900. PMID: 15707962
- Protein masking of a ribosomal RNA epitope is an early event in afferent deprivation-induced neuronal death. | Garden, GA., et al. 1995. Mol Cell Neurosci. 6: 293-310. PMID: 7496633
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
rRNA Antibody (Y10b) | sc-33678 | 200 µg/ml | $322.00 | |||
rRNA Antibody (Y10b): m-IgG Fc BP-HRP Bundle | sc-525542 | 200 µg Ab; 10 µg BP | $361.00 | |||
rRNA Antibody (Y10b) AC | sc-33678 AC | 500 µg/ml, 25% agarose | $424.00 | |||
rRNA Antibody (Y10b) HRP | sc-33678 HRP | 200 µg/ml | $322.00 | |||
rRNA Antibody (Y10b) FITC | sc-33678 FITC | 200 µg/ml | $336.00 | |||
rRNA Antibody (Y10b) PE | sc-33678 PE | 200 µg/ml | $349.00 | |||
rRNA Antibody (Y10b) Alexa Fluor® 488 | sc-33678 AF488 | 200 µg/ml | $364.00 | |||
rRNA Antibody (Y10b) Alexa Fluor® 546 | sc-33678 AF546 | 200 µg/ml | $364.00 | |||
rRNA Antibody (Y10b) Alexa Fluor® 594 | sc-33678 AF594 | 200 µg/ml | $364.00 | |||
rRNA Antibody (Y10b) Alexa Fluor® 647 | sc-33678 AF647 | 200 µg/ml | $364.00 | |||
rRNA Antibody (Y10b) Alexa Fluor® 680 | sc-33678 AF680 | 200 µg/ml | $364.00 | |||
rRNA Antibody (Y10b) Alexa Fluor® 790 | sc-33678 AF790 | 200 µg/ml | $364.00 |