rhodopsin 항체 (1D4) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 rhodopsin을 검출할것을 권장합니다.

rhodopsin 항체 (1D4): sc-57432

rhodopsin 항체 (1D4) is rated 4.4 out of 5 by 11.
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Datasheets

    • 안티-rhodopsin 항체 1D4 는 마우스 monoclonal IgG1 (kappa light chain) rhodopsin 항체 이며 200 µg/ml으로 제공합니다.
    • full length rhodopsin of bovine 의하고 제작된다
    • 안티-rhodopsin 항체 (1D4)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 rhodopsin 를 감지하는 데에 추천한다.; 이외에 bovine등 species와 반응할수 있습니다
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-rhodopsin 항체 (1D4)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • 강력추천하는 conventional secondary anti-mouse IgG reagents의 대체제품 보기m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP).
    • rhodopsin (1D4): sc-57432무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
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Would this antibody recognize the 1D4 tag in fusion protein in western blot?

Asked by: rogomca
Thank you for your question. This is a mouse monoclonal antibody that is recommended for detection of the protein rhodopsin. The clone name for this antibody is “1D4”, which is also known as the “1D4” epitope of rhodopsin. We have not tested this antibody for detection of any 1D4 fusion proteins.
Answered by: Technical Support
Date published: 2019-08-17

Can this mouse monoclonal antibody be used in IF or IHC on mouse tissue without background or non-specific staining issues?

Asked by: Anonymous
Thank you for your question. Yes, it can be used successfully for this application. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody rhodopsin (1D4): sc-57432 is currently available conjugated to HRP, PE, FITC, AF488 and AF647.
Answered by: Technical Support 10
Date published: 2017-01-09
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Rated 4 out of 5 by from Visualizing Endogenous Rhodopsin in RPE Cells Useful antibody for IF labeling of endogenous rhodopsin in RPE cells. Cells permeabilized for 30min in PBStween then blocked in 5%BSA. Antibody used at 1:200 overnight at 4 degrees.
Date published: 2020-02-14
Rated 4 out of 5 by from Antibody for IF I used this antibody on OCT embedded frozen retinal sections (mouse). Blocked for 1 hour with 10% goat serum buffer. Incubated with primary antibody overnight at the a concentration of 1:100. Incubated with secondary antibody (alexa fluor 568 anti-mouse) at a concentration of 1:1000. Staining was fairly robust but not completely consistent between all samples.
Date published: 2018-03-21
Rated 2 out of 5 by from Poor specificity in normal canine WB Antibody was used for WB with normal canine tissue. Very low specificity.
Date published: 2017-08-18
Rated 5 out of 5 by from Great reactivity. This antibody will give you strong and specific signals in your blotting and staining. It even cross-reacts with additional species. It has been published with over 15 times.
Date published: 2017-01-11
Rated 5 out of 5 by from Outstanding!! Great product for both western blot and ihc cell staining applications
Date published: 2017-01-05
Rated 4 out of 5 by from Decent I bought this antibody for WB of rhodopsin in Y79 cells. So far it works as advertised.
Date published: 2016-12-12
Rated 4 out of 5 by from Good specificity and signal strength with Good specificity and signal strength with some background in mouse eye and rat eye tissue extracts. -SCBT QC
Date published: 2015-06-01
Rated 5 out of 5 by from This antibody was used in a publication for This antibody was used in a publication for WB on human HEK 293 and ARPE-19 cells - SCBT Publication Review
Date published: 2015-04-04
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