RFX-B Antibody (E-4): sc-514873

RFX-B Antibody (E-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects RFX-B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RFX-B (E-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RFX-B protein plays a crucial role in the regulation of gene expression, particularly in the context of major histocompatibility complex (MHC) class II genes, which are essential for the immune response. Located primarily in the nucleus, RFX-B is part of a protein complex that binds to regulatory enhancer elements in the promoters of various genes, including those involved in immune function. This binding is vital for the transcriptional activation of MHC class II genes, especially in response to signals such as interferon-γ, which is critical for mounting an effective immune response. RFX family of proteins, including RFX-B, contains conserved structural features such as a DNA-binding domain and a dimerization region, which facilitate their function as transcription factors. By understanding RFX-B′s role in immune regulation, researchers can better explore therapeutic targets for autoimmune diseases and other conditions where immune response modulation is necessary.

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RFX-B Antibody (E-4) References:

1. Orientation and positional mapping of the subunits of the multicomponent transcription factors RFX and X2BP to the major histocompatibility complex class II transcriptional enhancer.  |  Westerheide, SD. and Boss, JM. 1999. Nucleic Acids Res. 27: 1635-41. PMID: 10075994
2. Structure of the winged-helix protein hRFX1 reveals a new mode of DNA binding.  |  Gajiwala, KS., et al. 2000. Nature. 403: 916-21. PMID: 10706293
3. Novel mutations within the RFX-B gene and partial rescue of MHC and related genes through exogenous class II transactivator in RFX-B-deficient cells.  |  Nagarajan, UM., et al. 2000. J Immunol. 164: 3666-74. PMID: 10725724
4. Functional organization of the hepatitis B virus enhancer.  |  Dikstein, R., et al. 1990. Mol Cell Biol. 10: 3683-9. PMID: 2355919
5. Assembly of functional regulatory complexes on MHC class II promoters in vivo.  |  Fontes, JD., et al. 1997. J Mol Biol. 270: 336-45. PMID: 9237901
6. The transcriptional activation and repression domains of RFX1, a context-dependent regulator, can mutually neutralize their activities.  |  Katan, Y., et al. 1997. Nucleic Acids Res. 25: 3621-8. PMID: 9278482
7. RFX1, a single DNA-binding protein with a split dimerization domain, generates alternative complexes.  |  Katan-Khaykovich, Y. and Shaul, Y. 1998. J Biol Chem. 273: 24504-12. PMID: 9733744
8. A gene encoding a novel RFX-associated transactivator is mutated in the majority of MHC class II deficiency patients.  |  Masternak, K., et al. 1998. Nat Genet. 20: 273-7. PMID: 9806546

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RFX-B Antibody (E-4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-RFX-B Antibody (E-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RFX-B Antibody (E-4) Specifications

Alternate Product Name: RFX-B

Product Clone Name: E-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-70

Epitope Target: RFX-B

Epitope Species: human

Product Reactivity/Detection: RFX-B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for RFX-B Antibody (E-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin