Rad52 항체 (F-7) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 Rad52을 검출할것을 권장합니다.

Rad52 항체 (F-7): sc-365341

Rad52 항체 (F-7) is rated 4.4 out of 5 by 16.
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데이터 시트

    • 안티-Rad52 항체 F-7 는 마우스 monoclonal IgG1 κ Rad52 항체, 22간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 Rad52을 항원으로 사용하였습니다.
    • 안티-Rad52 항체 (F-7)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 Rad52 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Rad52 항체 (F-7)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Rad52 (F-7): sc-365341무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for Rad52 Antibody (F-7) for WB and IHC(P) applications. These reagents are now offered in bundles with Rad52 Antibody (F-7) (see ordering information below).

Which conditions do you use to observe DNA damage-dependent Rad52 foci formation?

Asked by: isaw
Thank you for your question. Please contact our Technical Service Department if you have any specific questions about the data we show for this antibody on our website. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2019-11-18

What dilution do you use for IF?

Asked by: platon
Thank you for your question. We used this mouse monoclonal, Rad52 Antibody (F-7): sc-365341, at a dilution of 1:50 for immunofluorescent staining. The recommended range is 1:50-1:500, and optimal conditions should be determined by titrating the antibody in your application. A link to our recommended protocol for IF is provided below. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-16

Can you recommend a positive control to use with Rad52 (F-7): sc-365341 monoclonal antibody for Western blot?

Asked by: jenni
We recommend HCT 116 (sc-364175), SK-MEL-28 (sc-2236), HeLa (sc-2200), HEK 293 (sc-45136) or Raji (sc-364236) whole cell lysates to use as positive controls as we have Western blot data showing specific detection of Rad52 in these cell lines.
Answered by: Technical Support 15
Date published: 2017-01-11

What secondary is best to use with Rad52 (F-7): sc-365341 monoclonal antibody?

Asked by: jenni
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with Rad52 (F-7): sc-365341, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-11
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Rated 4 out of 5 by from Good for western blot This antibody is good for WB. After long term storage, it can get weeker. Longer exposure is recommended.
Date published: 2019-03-03
Rated 2 out of 5 by from Not appropriate for IF The antibody shows a pan-nuclear staining, but this is regardless of DNA damage treatment. I could not see any damage-specific foci formation.
Date published: 2018-03-16
Rated 4 out of 5 by from Worked well for WB This Ab worked great for WB. For IF, I did get signal from my staining but did not get nice foci unlike picture shown in website.
Date published: 2018-02-01
Rated 5 out of 5 by from Great antibody Good and clear wb detection in MCF7 cell lines using 1:200 in 70ug of protein.
Date published: 2017-11-29
Rated 5 out of 5 by from Excellent antibody!! We are getting into new areas of research and Rad52 is a protein of interest on three of our projects. The antibody provided to us by SantaCruz as a sample wroked well for us, for Rad52 positive and negative cell line.
Date published: 2017-11-17
Rated 5 out of 5 by from Great for WB Works great for WB at 1:1000 dilution in HeLa and 293T cells.
Date published: 2017-09-06
Rated 4 out of 5 by from Clean band Using film and ECL. Clean band detected, at recommended dilution, after several minute exposure. Long but reliable.
Date published: 2017-05-30
Rated 4 out of 5 by from Good antibody Nice sharp band in HeLa stable cell line after whole cell lysis. We used 1:1000 as dilution and we had signal after less than 20 secs in ChemiDoc using ECL.
Date published: 2017-04-13
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