PTPRH CRISPR/Cas9 KO Plasmid (h): sc-406293
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PTPRH CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PTPRH genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PTPRH CRISPR/Cas9 KO Plasmid (h) | sc-406293 | 20 µg | $397.00 |
Overview
PTPRH (protein tyrosine phosphatase receptor type H) is a transmembrane receptor-like phosphatase that modulates tyrosine phosphorylation signaling at the cell surface, shaping downstream pathways that control proliferation, adhesion, and cell motility. By counterbalancing receptor tyrosine kinase activity and associated MAPK and PI3K/AKT signaling outputs, PTPRH can influence growth factor responsiveness and epithelial cell behavior. Dysregulated PTPRH expression or activity has been linked to altered oncogenic signaling states and tumor-associated phenotypes in multiple cancer contexts, making it relevant for dissecting phosphorylation-dependent regulatory networks. As a membrane-associated phosphatase, it is also useful for studying signal integration between extracellular cues and intracellular kinase/phosphatase circuits.
PTPRH CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PTPRH gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PTPRH together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PTPRH open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PTPRH protein expression.
This CRISPR knockout system enables efficient generation of PTPRH-deficient cell models for investigation of PTPRH signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PTPRH exon(s) critical for PTPRH function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PTPRH genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PTPRH CRISPR/Cas9 KO Plasmid (h) and PTPRH CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PTPRH locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PTPRH HDR Plasmid (h) and PTPRH HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PTPRH homology arms to support homology-directed repair at defined PTPRH target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.