PSK2 CRISPR/Cas9 KO Plasmid (m): sc-432149
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PSK2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PSK2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PSK2 Antibody (22): sc-136094
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PSK2 CRISPR/Cas9 KO Plasmid (m) | sc-432149 | 20 µg | $397.00 |
Overview
Mouse Taok1 encodes the serine/threonine kinase PSK2, a STE20-family MAP3K that couples cytoskeletal cues to stress-responsive signaling. PSK2 participates in MAPK cascade regulation, including JNK and p38 pathways, and contributes to neuronal development, axon outgrowth, and synaptic organization through effects on microtubule and actin dynamics. Taok1 activity influences cell cycle and apoptosis-related programs, linking kinase signaling to cellular homeostasis. Dysregulation of TAOK1-associated networks has been implicated in neurodevelopmental phenotypes and other contexts where altered MAPK signaling and cytoskeletal remodeling are pathogenic mechanisms.
PSK2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Taok1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Taok1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Taok1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PSK2 protein expression.
This CRISPR knockout system enables efficient generation of Taok1-deficient cell models for investigation of PSK2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Taok1 exon(s) critical for PSK2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Taok1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PSK2 CRISPR/Cas9 KO Plasmid (m) and PSK2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Taok1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PSK2 HDR Plasmid (m) and PSK2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Taok1 homology arms to support homology-directed repair at defined Taok1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.