Date published: 2025-9-7

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PML 항체 (E-11): sc-377390

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데이터 시트
  • PML 항체 E-11 는 마우스 monoclonal IgG1 κ PML 항체, 26간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 PML에서 N-terminus에 위치한 27-63의 아미노산사이에 위치한 항원결정부와 특정결합합니다.
  • PML 항체 (E-11)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 all isoforms of PML 를 감지하는 데에 추천한다.
  • 항-PML 항체(E-11)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-377390 X, 200 µg/0.1 ml)이 있습니다.
  • PML (E-11): sc-377390무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • 현재 PML 항체(E-11)에 대해 선호하는 2차 검출 시약의 식별을 아직 완료하지 못했습니다. 이 작업은 진행 중입니다.

빠른 링크

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PML 항체(E-11)는 마우스, 쥐 및 인간 유래의 PML 단백질을 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgG1 κ 마우스 단일 클론 PML 항체(삼원 모티브(TRIM) 19 항체, TRIM19 항체 또는 전골수성 백혈병 항체라고도 칭함)입니다. PML 항체(E-11)는 비접합 항-PML 항체 형태뿐만 아니라 아가로스, HRP, PE, FITC 및 다중 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항-PML 항체 형태로도 사용할 수 있습니다. PML 단백질은 대체 스플라이싱으로 인해 세 가지 주요 전사 산물로 발현되는 징크 핑거 전사인자입니다. 인간 PML을 암호화하는 유전자는 염색체 15q22에 매핑됩니다. 레티노산 수용체 α(RARα) 유전자의 t(15;17) (q22;q11.2-q12) 염색체 전위는 거의 모든 급성 골수성 백혈병 사례에서 발생하며 PML/RARα 키메라 단백질의 발현을 초래합니다. PML/RARα 키메라를 발현하는 골수성 전구세포는 분화에 실패하고 세포 사멸이 감소하여 성장 속도가 증가합니다. PML/RARα는 레티노산 의존성 골수 분화에 필수적인 핵 공동 억제제(N-CoR)-히스톤 탈아세틸화 효소 복합체를 모집하여 골수 전구체를 변형시킵니다. 또한 PML/RARα는 유전자 과메틸화 및 침묵을 유도하여 궁극적으로 백혈병 생성을 촉진하기 위해 DNA 메틸전달효소를 모집합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

PML 항체 (E-11)

sc-377390
200 µg/ml
$316.00

PML 항체 (E-11) X

sc-377390 X
200 µg/0.1 ml
$316.00

PML 항체 (E-11) AC

sc-377390 AC
500 µg/ml, 25% agarose
$416.00

PML 항체 (E-11) HRP

sc-377390 HRP
200 µg/ml
$316.00

PML 항체 (E-11) FITC

sc-377390 FITC
200 µg/ml
$330.00

PML 항체 (E-11) PE

sc-377390 PE
200 µg/ml
$343.00

PML 항체 (E-11) Alexa Fluor® 488

sc-377390 AF488
200 µg/ml
$357.00

PML 항체 (E-11) Alexa Fluor® 546

sc-377390 AF546
200 µg/ml
$357.00

PML 항체 (E-11) Alexa Fluor® 594

sc-377390 AF594
200 µg/ml
$357.00

PML 항체 (E-11) Alexa Fluor® 647

sc-377390 AF647
200 µg/ml
$357.00

PML 항체 (E-11) Alexa Fluor® 680

sc-377390 AF680
200 µg/ml
$357.00

PML 항체 (E-11) Alexa Fluor® 790

sc-377390 AF790
200 µg/ml
$357.00

PML (E-11) 중화펩타이드

sc-377390 P
100 µg/0.5 ml
$68.00

In data sheet, "Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS ...". This mean that PML(E-11) antibody has no heavy chain? Is it possible to use this antibody for immunoprecipitation where Fc part has to be bound to Protein G?

Asked by: snkm
Thank you for your question. The antibody is a complete antibody, the light chain type is Kappa light chain and contains heavy chain.PML Antibody (E-11) is recommended for detection of all isoforms of PML of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA.Thank you.
Answered by: Jiajun Z
Date published: 2023-11-20

Dear responsible, I would like to know if you have evidence that the antibody recognize the mouse Pml protein as well?

Asked by: Anonymous
Thank you for your question. Anti-PML Antibody (E-11) is recommended for detection of all isoforms of PML of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA.
Answered by: Tech Support Europe
Date published: 2020-11-02

Hi, Does this antibody also work for fluorescence in situ hybridization? Thank you.

Asked by: yin978
Thank you for your question. This antibody has not been tested for its viability in in situ hybridization experiments.
Answered by: Tech Support Europe
Date published: 2020-07-14

What is the recommended starting dilution for Western blot for PML (E-11): sc-377390?

Asked by: jenni
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-377390.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 8
Date published: 2017-01-26
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Rated 5 out of 5 by from 很厉害很高兴申请到,我觉得非常开心非常开心,开心非常开心非常开心非常开心非常开心非常开心非常开心非常开心啊。
Date published: 2018-11-15
Rated 1 out of 5 by from It does not work for mouseTry WB and IF by mouse breast cancer cell lines, No signal for both method.
Date published: 2018-02-20
Rated 4 out of 5 by from Works well in immunofluorescenceIt works very well in my immunofluorescence assay.
Date published: 2018-02-09
Rated 5 out of 5 by from Works for different applicationsWe have tried this antibody on western blot and immunofluorescent microscopy and it worked well.
Date published: 2018-01-24
Rated 3 out of 5 by from Not very wellWe used this antibody for WB, and could not see specific bands. But it worked well for IF in formaldehyde-fixed HMEC1 cells.
Date published: 2017-12-05
Rated 1 out of 5 by from uslessI used this antibody to detet PML protein in WCL from L929 cells in a ratio 1:500. however, this antibody worked badly.
Date published: 2017-10-28
Rated 3 out of 5 by from OKI could detect nuclear PML using A549 cell nuclear extract, but it was weak.
Date published: 2017-10-13
Rated 1 out of 5 by from Antibody specificity is not good!We used the antibody to do the first WB, found no clear band. And then do the immunofluorescence, the fluorescence results are also poor, almost no fluorescent signal.
Date published: 2017-09-09
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PML 항체 (E-11) is rated 3.8 out of 5 by 16.
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