PME-1 Antibody (E-8): sc-55472

PME-1 Antibody (E-8) is a mouse monoclonal IgG2a antibody that detects protein phosphatase methylesterase-1 (PME-1) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PME-1 plays a crucial role in the regulation of protein phosphatase 2A (PP2A), a key enzyme involved in cell growth inhibition and cell cycle regulation, by catalyzing the demethylation and inactivation of this phosphatase. The ability of PME-1 to demethylate the catalytic subunit of PP2A is vital for maintaining proper cellular signaling and function, as dysregulation of PP2A activity is associated with various diseases, including cancer. PME-1 is conserved across species from yeast to humans and features a catalytic triad similar to that found in lipases, with an activated serine serving as the nucleophile at its active site. This structural similarity underscores PME-1′s importance in cellular processes and highlights PME-1′s potential as a therapeutic target. Additionally, the covalent modifications of carboxymethylation and demethylation that PME-1 mediates are influenced by electrostatic interactions at specific residues or metal ions near the active site, further emphasizing the complexity and significance of PME-1′s regulatory functions in eukaryotic cells.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PME-1 Antibody (E-8) References:

1. A protein phosphatase methylesterase (PME-1) is one of several novel proteins stably associating with two inactive mutants of protein phosphatase 2A.  |  Ogris, E., et al. 1999. J Biol Chem. 274: 14382-91. PMID: 10318862
2. PME-1 protects extracellular signal-regulated kinase pathway activity from protein phosphatase 2A-mediated inactivation in human malignant glioma.  |  Puustinen, P., et al. 2009. Cancer Res. 69: 2870-7. PMID: 19293187
3. Protein Phosphatase 2A and Its Methylation Modulating Enzymes LCMT-1 and PME-1 Are Dysregulated in Tauopathies of Progressive Supranuclear Palsy and Alzheimer Disease.  |  Park, HJ., et al. 2018. J Neuropathol Exp Neurol. 77: 139-148. PMID: 29281045
4. Hydrogen peroxide redistributes the localization of protein phosphatase methylesterase 1.  |  Tang, S., et al. 2018. Life Sci. 213: 166-173. PMID: 30340029
5. Glycogen synthase kinase-3β suppresses the expression of protein phosphatase methylesterase-1 through β-catenin.  |  Jin, N., et al. 2019. Aging (Albany NY). 11: 9672-9688. PMID: 31714894
6. Phosphoproteome and drug-response effects mediated by the three protein phosphatase 2A inhibitor proteins CIP2A, SET, and PME-1.  |  Kauko, O., et al. 2020. J Biol Chem. 295: 4194-4211. PMID: 32071079
7. Inhibition of protein phosphatase methylesterase 1 dysregulates MAP kinase signaling and attenuates muscle cell differentiation.  |  Labuzan, SA., et al. 2020. Gene. 739: 144515. PMID: 32112987
8. Reduced Expression of the PP2A Methylesterase, PME-1, or the PP2A Methyltransferase, LCMT-1, Alters Sensitivity to Beta-Amyloid-Induced Cognitive and Electrophysiological Impairments in Mice.  |  Staniszewski, A., et al. 2020. J Neurosci. 40: 4596-4608. PMID: 32341098
9. Hyperphosphorylation of Tau Due to the Interference of Protein Phosphatase Methylesterase-1 Overexpression by MiR-125b-5p in Melatonin Receptor Knockout Mice.  |  Zhao, H., et al. 2021. Int J Mol Sci. 22: PMID: 34769281
10. The luciferase-based in vivo protein-protein interaction assay revealed that CHK1 promotes PP2A and PME-1 interaction.  |  Ando, S., et al. 2024. J Biol Chem. 300: 107277. PMID: 38588804

The preferred PME-1 antibody is PME-1 Antibody (B-12): sc-25278.