Pirh2 Antibody (D-12): sc-374505
- Pirh2 Antibody (D-12) is a mouse monoclonal IgG2b κ Pirh2 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against a peptide mapping within an internal region of Pirh2 of human origin
- Pirh2 Antibody (D-12) is recommended for detection of Pirh2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Pirh2 Antibody (D-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-374505 X, 200 µg/0.1 ml)
- m-IgG2b BP-HRP is the preferred secondary detection reagent for Pirh2 Antibody (D-12) for WB applications. This reagent is now offered in a bundle with Pirh2 Antibody (D-12) (see ordering information below).
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Pirh2 Antibody (D-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects Pirh2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Pirh2 (D-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Pirh2, also known as Androgen receptor N-terminal-interacting protein (ARNIP), ZN363, or CHIMP, plays a critical role in regulating the p53 tumor suppressor pathway through ubiquitin-protein ligase activity, which promotes p53 degradation. This interaction is significant because p53 is a key regulator of cell cycle and apoptosis, and loss of function is associated with various malignancies. The gene encoding Pirh2 is located on chromosome 4q21.1, and expression is tightly regulated by p53 itself, creating a feedback loop that maintains cellular homeostasis. Notably, increased levels of Pirh2 lead to decreased p53, while reduced Pirh2 expression results in elevated p53 levels, highlighting Pirh2′s role as a negative regulator of p53 function alongside MDM2. This intricate balance underscores Pirh2′s importance in cancer biology and potential as a therapeutic target.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Pirh2 Antibody (D-12) References:
- Cloning and characterization of an androgen receptor N-terminal-interacting protein with ubiquitin-protein ligase activity. | Beitel, LK., et al. 2002. J Mol Endocrinol. 29: 41-60. PMID: 12200228
- Pirh2, a p53-induced ubiquitin-protein ligase, promotes p53 degradation. | Leng, RP., et al. 2003. Cell. 112: 779-91. PMID: 12654245
- The ubiquitin ligase COP1 is a critical negative regulator of p53. | Dornan, D., et al. 2004. Nature. 429: 86-92. PMID: 15103385
- The p53 paddy wagon: COP1, Pirh2 and MDM2 are found resisting apoptosis and growth arrest. | Corcoran, CA., et al. 2004. Cancer Biol Ther. 3: 721-5. PMID: 15280670
- Expression of Pirh2, a newly identified ubiquitin protein ligase, in lung cancer. | Duan, W., et al. 2004. J Natl Cancer Inst. 96: 1718-21. PMID: 15547185
- dapk1, encoding an activator of a p19ARF-p53-mediated apoptotic checkpoint, is a transcription target of p53. | Martoriati, A., et al. 2005. Oncogene. 24: 1461-6. PMID: 15608685
- A new human gene hNTKL-BP1 interacts with hPirh2. | Zhang, L., et al. 2005. Biochem Biophys Res Commun. 330: 293-7. PMID: 15781263
- Transactivation-dependent and -independent regulation of p73 stability. | Dulloo, I. and Sabapathy, K. 2005. J Biol Chem. 280: 28203-14. PMID: 15919663
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pirh2 Antibody (D-12) | sc-374505 | 200 µg/ml | $322.00 | |||
Pirh2 Antibody (D-12): m-IgG2b BP-HRP Bundle | sc-548743 | 200 µg Ab; 10 µg BP | $361.00 | |||
Pirh2 Antibody (D-12) X | sc-374505 X | 200 µg/0.1 ml | $322.00 | |||
Pirh2 Antibody (D-12) AC | sc-374505 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Pirh2 Antibody (D-12) HRP | sc-374505 HRP | 200 µg/ml | $322.00 | |||
Pirh2 Antibody (D-12) FITC | sc-374505 FITC | 200 µg/ml | $336.00 | |||
Pirh2 Antibody (D-12) PE | sc-374505 PE | 200 µg/ml | $349.00 | |||
Pirh2 Antibody (D-12) Alexa Fluor® 488 | sc-374505 AF488 | 200 µg/ml | $364.00 | |||
Pirh2 Antibody (D-12) Alexa Fluor® 546 | sc-374505 AF546 | 200 µg/ml | $364.00 | |||
Pirh2 Antibody (D-12) Alexa Fluor® 594 | sc-374505 AF594 | 200 µg/ml | $364.00 | |||
Pirh2 Antibody (D-12) Alexa Fluor® 647 | sc-374505 AF647 | 200 µg/ml | $364.00 | |||
Pirh2 Antibody (D-12) Alexa Fluor® 680 | sc-374505 AF680 | 200 µg/ml | $364.00 | |||
Pirh2 Antibody (D-12) Alexa Fluor® 790 | sc-374505 AF790 | 200 µg/ml | $364.00 | |||
Pirh2 (D-12) Neutralizing Peptide | sc-374505 P | 100 µg/0.5 ml | $69.00 |