human origin의 PICOT에서 N-terminus에 위치한 31-69의 아미노산사이에 위치한 항원결정부와 특정결합합니다.
안티-PICOT 항체 (D-10)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 PICOT 를 감지하는 데에 추천한다.
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-PICOT 항체 (D-10)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PICOT Antibody (D-10) for WB applications. These reagents are now offered in bundles with PICOT Antibody (D-10) (see ordering information below).
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
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Labware ships FedEx Ground free of charge to the contiguous US
PICOT Antibody (D-10) is a high quality monoclonal PICOT antibody (also designated PICOT antibody) suitable for the detection of the PICOT protein of mouse, rat and human origin. PICOT Antibody (D-10) is available as both the non-conjugated anti-PICOT antibody form, as well as multiple conjugated forms of anti-PICOT antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. PICOT, also known as GLRX3 (glutaredoxin 3), GRX3, GRX4, GLRX4, HUSSY-22, TXNL2 or TXNL3, is a 335 amino acid protein that contains one thioredoxin domain and two glutaredoxin domains. Localized to the cytoplasm and the cell cortex, PICOT is thought to play a role in regulating the thioredoxin system and may weakly interact with PKC θ (protein kinase C theta). Through its ability to regulate the thioredoxin pathway, PICOT inhibits cardiac hypertrophy (a thickening of the heart muscle usually caused by high blood pressure) by negatively regulating NFAT (Nuclear factor of activated T-cells) signaling. Although PICOT contains one thioredoxin domain, it lacks the two redox-reactive cysteines that are required for catalytic activity, suggesting that PICOT lacks thioredoxin function. PICOT is expressed in testis, heart and spleen with lower levels detected in thymus, lung, colon, placenta and small intestine.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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