Phosphatase Inhibitor Cocktail A L-isozymes of alkaline phosphatase 및 Ser/Thr protein phosphatases 억제제

Phosphatase Inhibitor Cocktail A

  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 122ms
  • REVIEWS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;
Phosphatase Inhibitor Cocktail A is rated 4.75 out of 5 by 4.
loading
5
3
4
1
3
0
2
0
1
0
Alternate Names: Phosphatase Inhibitor Cocktail I
Application Phosphatase Inhibitor Cocktail A is an inhibitor of L-isozymes of alkaline phosphatase and ser/thr protein phosphatases
연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.
* Refer to Certificate of Analysis for lot specific data (including water content).
loading

Phosphatase Inhibitor Cocktail A effectively inhibits L-isozymes of alkaline phosphatase and serine/threonine protein phosphatases, such as the PP1 (protein phosphatase 1) and PP2A) protein phosphatase 2a) proteins. Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of modifying the proteins present in the extract. The best way to improve the yield of intact proteins is to add inhibitors of these enzymes known to be present in the source material. Recommended for use with tissue and cell extracts, including extracts containing detergents.
Each vial contains 2.5 mM (-)-p-Bromotetramisole oxalate, 500 µM Cantharidin and 500 nM Microcystin-LR in 1 ml DMSO. Because this product is not available for sale outside of the United States, please see Phosphatase Inhibitor Cocktail C (sc-45065) as an alternate product.


References

1. Nishiwaki, S., et al. 1991. FEBS Lett. 279: 115-118. PMID: 1847341
2. Ilkovitch, D., et al. 2008. Cancer Res. 68: 2427-2435. PMID: 18381451
3. Wagner, M.W., et al. 2008. J. Biol. Chem. 283: 21382-21393. PMID: 18480061
4. Jain, M.K. 1982. Handbook of Enzyme Inhibitors. New York: John Wiley and Sons, Pg. 222, 334.

사용법 :
• Recommended usage is 100-fold dilution.

• One ml of Phosphatase Inhibitor Cocktail A will inhibit phosphatase activities found in the 100,000 x g supernatant from human placenta, cow liver, rabbit muscle, A431 or Jurkat cell extracts at a protein concentration of approximately 5 mg/ml.

• One ml of Phosphatase Inhibitor Cocktail A solution is used to prepare 100 ml of supernatant that contains a maximum of 500 mg of protein. Therefore, 1 ml of cocktail should be added per 500 mg of protein extracted from the tissue used, or 1 ml of cocktail solution per 100 ml of extraction buffer.
Physical State :
Liquid
저장 :
Store at -20° C
연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

SDS(MSDS)다운로드

분석증명서

PDF문서를 열람,
프린트와 코멘트를 작성하려면 Adobe Acrobat Reader가 필요합니다.

Phosphatase Inhibitor Cocktail A  논문정보

다른 사용자의 Phosphatase Inhibitor Cocktail A사용법보기. 클릭하여 PubMed entry보기 .

인용 1 to 10 of 64 합계

PMID: # 35252945  Bray, D.|Hook, H.|Zhao, R.|Keenan, JL.|Penvose, A.|Osayame, Y.|Mohaghegh, N.|Chen, X.|Parameswaran, S.|Kottyan, LC.|Weirauch, MT.|Siggers, T.| et al. 2022. Cell Genom. 2:

PMID: # 35302352  Cornejo, NR.|Amofah, B.|Lipinski, A.|Langlais, PR.|Ghosh, I.|Jewett, JC.| et al. 2022. Biochemistry.

PMID: # 35213584  Manno, C.|Tammineni, E.|Figueroa, L.|Marty, I.|Ríos, E.| et al. 2022. PLoS One. 17: e0264146.

PMID: # 35311588  Wang, Z.|Xiong, L.|Xiong, Q.| et al. 2022. Mol Cell Biol. 42: e0052321.

PMID: # 35361260  Quwaider, D. et al. 2022. Exp Hematol Oncol. 11: 18.

PMID: # 33624556  Taylor, CM.|Kasztan, M.|Sedaka, R.|Molina, PA.|Dunaway, LS.|Pollock, JS.|Pollock, DM.| et al. 2021. Am J Physiol Regul Integr Comp Physiol. 320: R630-R640.

PMID: # 34879284  Yang, Y.|He, Y.|Liu, H.|Zhou, W.|Wang, C.|Xu, P.|Cai, X.|Liu, H.|Yu, K.|Pei, Z.|Hyseni, I.|Fukuda, M.|Tong, Q.|Xu, J.|Sun, Z.|O'Malley, BW.|Xu, Y.| et al. 2021. Cell Rep. 37: 110075.

PMID: # 33500244  Duy, C.|Li, M.|Teater, M.|Meydan, C.|Garrett-Bakelman, FE.|Lee, TC.|Chin, CR.|Durmaz, C.|Kawabata, KC.|Dhimolea, E.|Mitsiades, CS.|Doehner, H.|D'Andrea, RJ.|Becker, MW.|Paietta, EM.|Mason, CE.|Carroll, M.|Melnick, AM.| et al. 2021. Cancer Discov.

PMID: # 34526526  Li, Y.|Mao, P.|Basenko, EY.|Lewis, Z.|Smerdon, MJ.|Czaja, W.| et al. 2021. Sci Rep. 11: 18393.

PMID: # 33441552  Wu, CL.|Dicks, A.|Steward, N.|Tang, R.|Katz, DB.|Choi, YR.|Guilak, F.| et al. 2021. Nat Commun. 12: 362.

인용 1 to 10 of 64 합계
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 107ms
  • QUESTIONS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 6ms
  • REVIEWS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;