Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Phocein CRISPR/Cas9 KO Plasmid (h): sc-418293
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Phocein CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Phocein genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Phocein Antibody (D-5): sc-137229
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Phocein CRISPR/Cas9 KO Plasmid (h) | sc-418293 | 20 µg | $397.00 |
Overview
MOB4 encodes the human protein phocein, a conserved scaffold component implicated in regulation of Hippo pathway signaling through association with STRIPAK/PP2A-linked complexes and related serine/threonine kinase networks. Phocein contributes to coordination of cell polarity, cytoskeletal organization, and vesicle trafficking processes that influence proliferation and migration. Through these signaling hubs, MOB4 has been connected to control of tissue growth and stress-responsive signaling nodes that are frequently perturbed in cancer-associated pathways. Dissecting phocein function supports mechanistic studies of pathway crosstalk affecting YAP/TAZ activity, phosphorylation dynamics, and context-dependent cell fate decisions.
Phocein CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MOB4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MOB4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MOB4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Phocein protein expression.
This CRISPR knockout system enables efficient generation of MOB4-deficient cell models for investigation of Phocein signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MOB4 exon(s) critical for Phocein function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MOB4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Phocein CRISPR/Cas9 KO Plasmid (h) and Phocein CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MOB4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Phocein HDR Plasmid (h) and Phocein HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MOB4 homology arms to support homology-directed repair at defined MOB4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.