Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Peroxin 10 CRISPR/Cas9 KO Plasmid (h): sc-406226
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Peroxin 10 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Peroxin 10 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Peroxin 10 Antibody (Y-2D3): sc-134419
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Peroxin 10 CRISPR/Cas9 KO Plasmid (h) | sc-406226 | 20 µg | $397.00 |
Overview
PEX10 encodes peroxin 10, a RING finger E3 ubiquitin ligase embedded in the peroxisomal membrane that is essential for peroxisome biogenesis and matrix protein import. As part of the peroxisomal ubiquitination machinery with PEX2 and PEX12, PEX10 helps regulate recycling of the PEX5 import receptor and supports peroxisomal metabolic pathways including very-long-chain fatty acid β-oxidation and reactive oxygen species homeostasis. Disruption of PEX10 impairs peroxisome assembly and compromises lipid metabolism and redox balance, processes central to cellular stress responses and organelle crosstalk. Pathogenic variants in PEX10 are linked to peroxisome biogenesis disorders within the Zellweger spectrum, making it a relevant target for studying mechanisms of organelle dysfunction.
Peroxin 10 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PEX10 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PEX10 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PEX10 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Peroxin 10 protein expression.
This CRISPR knockout system enables efficient generation of PEX10-deficient cell models for investigation of Peroxin 10 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PEX10 exon(s) critical for Peroxin 10 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PEX10 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Peroxin 10 CRISPR/Cas9 KO Plasmid (h) and Peroxin 10 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PEX10 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Peroxin 10 HDR Plasmid (h) and Peroxin 10 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PEX10 homology arms to support homology-directed repair at defined PEX10 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.