Date published: 2025-9-8

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CD31/PECAM-1 항체 (H-3): sc-376764

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데이터 시트
  • CD31/PECAM-1 항체 H-3 는 마우스 monoclonal IgG1 κ CD31/PECAM-1 항체, 231간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 항원결정부는 mouse origin의 PECAM-1의 내부에 위치한 699-727의 아미노산에 해당됩니다.
  • CD31/PECAM-1 항체 (H-3)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 PECAM-1 를 감지하는 데에 추천한다.
  • 항-CD31/PECAM-1 항체(H-3)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • CD31/PECAM-1 (H-3): sc-376764무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 는 CD31/PECAM-1 항체 (H-3) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은CD31/PECAM-1 항체(H-3) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다)

빠른 링크

더보기

PECAM-1 항체(H-3)는 마우스, 쥐 및 인간 유래의 PECAM-1 단백질을 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgG1 κ 마우스 모노클로날 PECAM-1 항체(CD31 항체 또는 엔도CAM 항체라고도 지정됨)입니다. PECAM-1 항체(H-3)는 비접합 항-PECAM-1 항체 형태뿐만 아니라 아가로스, HRP, PE, FITC 및 다중 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항-PECAM-1 항체 형태로도 사용할 수 있습니다. 세포 접착 분자는 성장하는 동안 세포와 세포 간의 상호작용에 관여하는 밀접하게 관련된 세포 표면 당단백질 계열로, 배아 발생과 발달에 중요한 역할을 하는 것으로 알려져 있습니다. 신경세포 접착 분자(NCAM)의 발현은 신경모세포종, 횡문근육종, 윌름스 종양, 유잉 육종 및 일부 원시 골수성 악성 종양을 포함한 다양한 인간 종양에서 관찰됩니다. CD54라고도 하는 세포 내 접착 분자-1(ICAM-1)은 면역 글로불린 슈퍼 계열의 필수 막 단백질이며 β2/α1 및 β2/αM 인테그린을 인식합니다. CD31이라고도 하는 PECAM-1(혈소판/내피 세포 접착 분자-1)은 단핵구, 호중구, 혈소판 및 T 세포의 일부 집단의 세포 표면에 발현되는 당단백질입니다. VCAM-1(혈관 세포 접착 분자-1)은 IL-1, 종양 괴사인자(TNF), 지질 다당류(LPS) 등의 염증성 사이토카인에 의해 인간 내피 세포에서 유도되는 접착 분자로 처음 확인되었습니다. KALIG 유전자는 신경 세포 부착 분자(NCAM)와 유사한 단백질을 암호화하며 이차성 성선 기능 저하증을 동반한 무감각증인 칼만 증후군 환자의 66%에서 결손되어 있습니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

CD31/PECAM-1 항체 (H-3)

sc-376764
200 µg/ml
$316.00

CD31/PECAM-1 (H-3): m-IgG Fc BP-HRP 번들

sc-527547
200 µg Ab; 10 µg BP
$354.00

CD31/PECAM-1 (H-3): m-IgG1 BP-HRP 번들

sc-532920
200 µg Ab; 20 µg BP
$354.00

CD31/PECAM-1 항체 (H-3) AC

sc-376764 AC
500 µg/ml, 25% agarose
$416.00

CD31/PECAM-1 항체 (H-3) HRP

sc-376764 HRP
200 µg/ml
$316.00

CD31/PECAM-1 항체 (H-3) FITC

sc-376764 FITC
200 µg/ml
$330.00

CD31/PECAM-1 항체 (H-3) PE

sc-376764 PE
200 µg/ml
$343.00

CD31/PECAM-1 항체 (H-3) Alexa Fluor® 488

sc-376764 AF488
200 µg/ml
$357.00

CD31/PECAM-1 항체 (H-3) Alexa Fluor® 546

sc-376764 AF546
200 µg/ml
$357.00

CD31/PECAM-1 항체 (H-3) Alexa Fluor® 594

sc-376764 AF594
200 µg/ml
$357.00

CD31/PECAM-1 항체 (H-3) Alexa Fluor® 647

sc-376764 AF647
200 µg/ml
$357.00

CD31/PECAM-1 항체 (H-3) Alexa Fluor® 680

sc-376764 AF680
200 µg/ml
$357.00

CD31/PECAM-1 항체 (H-3) Alexa Fluor® 790

sc-376764 AF790
200 µg/ml
$357.00

CD31/PECAM-1 (H-3) 중화펩타이드

sc-376764 P
100 µg/0.5 ml
$68.00

buongiorno, desidererei sapere quale secondario usare con questo anticorpo

Asked by: AlePri
Thank you for your question. CD31/PECAM-1 Antibody (H-3) is a mouse monoclonal IgG1 κ antibody that can be detected using any of our anti-kappa, anti-Fc, or anti-IgG1 secondary antibodies. Please contact technical service for further information on what would be suitable for your particular experimental setup.
Answered by: Tech Support Europe
Date published: 2025-04-02

What is the suitable antigen retrieval method for this antibody?

Asked by: DiVa03
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext .1, allowing for a more interactive discussion of this and other related questions
Answered by: Blake J
Date published: 2021-09-27

Are trial samples for this antibody offered?

Asked by: james001
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Hola, para este anticuerpo es mejor para bloqueo leche, BSA %??, y que porcentaje de tween? y para lavar, PBS o TBS? Hello, for this antibody is better for blocking milk, BSA, %?? and what percentage of tween? and to wash, PBS or TBS?

Asked by: MARIACONSUELO
Thank you for your question. We recommend blocking with 5% milk or 3% BSA with 0.05% Tween-20 for Western blot. We wash with TBS but PBS would work to. If you have additional questions, please contact Technical Support at 1800-457-3801 option 2, by emailing scbt@scbt.com or by live chat on our website at www.scbt.com so we may further assist you.
Answered by: Santa Cruz TS
Date published: 2021-07-07

Hi, can i get a testing aliquot of this antibody for western blot? I need to examine PECAM protein level in human endothelial cells and would like to try out the antibody before we purchase a whole vial. 

Asked by: Yitong
Thank you for your question. Please get in touch with our technical service so we can arrange providing a free sample of this antibody.
Answered by: Tech Support Europe
Date published: 2020-07-08

Hi, can PECAM-1 Anticuerpo (H-3) sc-376764 be used with mouse tissues in histochemistry without any background?

Asked by: Fernandog
Thank you for your question. PECAM Antibody (H-3): sc-376764 is suitable for the detection of PECAM in mouse samples. Depending on the sample, antigen demasking may be necessary. You can find our recommended protocol for immunofluorescence, IHC and different antigen demasking methods by following this link: https://www.scbt.com/scbt/de/resources/protocols/immunofluorescence-cell-staining
Answered by: Tech Service
Date published: 2019-05-20

Is PECAM1 antibody (H3) conjugated to Alexa Fluor 594 stable to fixation and the subsequent solvents such as ethanol and xylene? If kept dark will it still fluoresce?

Asked by: Barb
Thank you for your question. Yes, the Alexa Fluor 594 conjugated antibody is a bright, red-fluorescent dye that provides stable imaging for immunofluorescent staining applications. Alexa Fluor 594 dye is pH-insensitive over a wide molar range. We recommend to perform all steps in the dark. Please see our recommended protocol below for details. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2018-12-12

Is this antibody suitable for IHC of frozen sections, specifically frozen rat brain?

Asked by: harding
Thank you for your question. PECAM-1 (H-3): sc-376764 is recommended for use with frozen tissue sections by IHC. PECAM-1 (H-3) has been demonstrated in several publications to stain rat tissue by IHC.
Answered by: Technical Support
Date published: 2018-12-05
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Rated 5 out of 5 by from Ottimo funzionamento!Ho provato questo ab in WB su lisati da tessuto murino e funziona molto bene!
Date published: 2025-06-04
Rated 4 out of 5 by from It works in IFI tried this antibody in frozen section of mouse mesenteric arteries and it worked at 1:50 dilution
Date published: 2023-03-08
Rated 4 out of 5 by from Works wellI used the dilution with 1:100 for IF in mouse brain, without any antigen retrieval processes. When I tried the antigen retrieval with Hcl/ heating, it stained but not as good as the tissue stained normally. If you are doing the IF, then I recommend not to use the antigen retrieval.
Date published: 2022-11-15
Rated 1 out of 5 by from Did not work on rat brain tissueWe tested this antibody at a range of dilutions on frozen rat brain tissue fixed with either methanol or 4% paraformaldehyde and observed no staining.
Date published: 2022-01-20
Rated 5 out of 5 by from Very good antibodyWe have used this antibody in WB and worked very well at 1:1000.
Date published: 2020-11-30
Rated 4 out of 5 by from A-FABP (B-4) antibody sc-271529It is a great Ab which I got as a free sample five month ago.
Date published: 2019-12-06
Rated 5 out of 5 by from Excellent specificitySanta Cruz Biotechnology gives us a sample of its well characterized monoclonal PECAM-1 antibody two months ago and we have obtained excellent results
Date published: 2019-02-18
Rated 5 out of 5 by from Great for WBIt works very well on HeLa cell extract. Dilution 1:500.
Date published: 2018-10-08
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CD31/PECAM-1 항체 (H-3) is rated 4.3 out of 5 by 43.
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