PCB Antibody (E-5): sc-365672

PCB Antibody (E-5) is a mouse monoclonal IgG2b antibody that detects pyruvate carboxylase in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-pyruvate carboxylase antibody (E-5) recognizes pyruvate carboxylase, a crucial mitochondrial enzyme responsible for catalyzing the conversion of pyruvate to oxaloacetate, a key step in gluconeogenesis and the citric acid cycle. This enzymatic function is vital for maintaining glucose homeostasis and energy production, particularly in tissues with high metabolic demands, such as the liver and brain. Proper functioning of pyruvate carboxylase is essential, as deficiencies can lead to severe metabolic disorders, including lactic acidosis and hypoglycemia, which can have profound effects on neurological development and function. PCB gene, located on the long arm of chromosome 11, is subject to mutations that can disrupt activity, highlighting the importance of pyruvate carboxylase in metabolic regulation and potential as a therapeutic target.

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PCB Antibody (E-5) References:

1. Glutamine-, glutamine synthetase-, glutamate dehydrogenase- and pyruvate carboxylase-immunoreactivities in the rat dorsal root ganglion and peripheral nerve.  |  Miller, KE., et al. 2002. Brain Res. 945: 202-11. PMID: 12126882
2. Distribution of pyruvate carboxylase along the rat nephron: an immunological and enzymatic study.  |  Salto, R., et al. 1991. Kidney Int. 39: 1162-7. PMID: 1895670
3. Electron microscopic localization of pyruvate carboxylase in rat liver and Saccharomyces cerevisiae by immunogold procedures.  |  Rohde, M., et al. 1991. Arch Biochem Biophys. 290: 197-201. PMID: 1898090
4. Analyzing clonal variation of monoclonal antibody-producing CHO cell lines using an in silico metabolomic platform.  |  Ghorbaniaghdam, A., et al. 2014. PLoS One. 9: e90832. PMID: 24632968
5. Pyruvate carboxylase from Pseudomonas citronellolis: shape of the enzyme, and localization of its prosthetic biotin group by electron microscopic affinity labeling.  |  Fuchs, J., et al. 1988. FEBS Lett. 231: 102-6. PMID: 3360116
6. Immunochemical studies with soluble and mitochondrial pyruvate carboxylase activities from rat tissues.  |  Ballard, FJ., et al. 1970. Biochem J. 119: 735-42. PMID: 5493507
7. Immunofluorescent localization of acetyl CoA carboxylase, fatty acid synthetase and pyruvate carboxylase during the adipocyte conversion of 3T3 fibroblasts.  |  Gratzner, HG., et al. 1980. Cell Biol Int Rep. 4: 497-508. PMID: 6105017
8. A simple method for the preparation of antibodies to the mitochondrial biotin-dependent carboxylases.  |  McKeon, C. and Wolf, B. 1982. Experientia. 38: 281-2. PMID: 6120854
9. Regulation of the synthesis and degradation of pyruvate carboxylase in 3T3-L1 cells.  |  Freytag, SO. and Utter, MF. 1983. J Biol Chem. 258: 6307-12. PMID: 6343373
10. Biochemical characterization of biotin-responsive multiple carboxylase deficiency: heterogeneity within the bio genetic complementation group.  |  Feldman, GL., et al. 1981. Am J Hum Genet. 33: 692-701. PMID: 6794361

The preferred PCB antibody is PCB Antibody (H-2): sc-271493.