Date published: 2025-9-11

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p35 항체 (4G11): sc-293184

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데이터 시트
  • p35 항체 4G11 는 마우스 monoclonal IgG2a κ p35 항체, 6간행물에 인용, 이며 100 µg/ml으로 제공합니다.
  • 아미노산 208-307에 대해 제기된 부분 길이를 나타내는 재조합 p35 단백질 human 기원
  • WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 p35을 검출할것을 권장합니다.
  • p35 (4G11): sc-293184무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • 현재 p35 항체(4G11)에 대해 선호하는 2차 검출 시약의 식별을 아직 완료하지 못했습니다. 이 작업은 진행 중입니다.

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p35 항체(4G11)는 마우스, 랫트, 인간에서 유래된 p35 단백질을 웨스턴 블롯팅(WB), 면역침전법(IP), 면역형광법(IF), 효소결합 면역흡착 분석법(ELISA)을 통해 검출하는 마우스 단일클론 IgG2a 카파 경쇄 항체입니다. ISA). 항-p35 항체(4G11)는 비접합 형태로 제공됩니다. CDK5R1로도 알려진 p35 단백질은 적절한 신경 기능과 발달에 필수적인 사이클린 의존성 키나아제 5(Cdk5)를 조절하는 데 중요한 역할을 합니다. Cdk5는 특히 뇌에서 중요합니다. 뇌에서 Cdk5는 신경세포 이동, 시냅스 가소성, 세포주기 조절과 같은 과정을 조절합니다. p35와 Cdk5의 상호작용은 매우 중요합니다. p35는 Cdk5의 키나아제 활성을 활성화하여 Cdk5가 이러한 중요한 신경세포 과정에 관여하는 다양한 기질을 인산화할 수 있도록 하기 때문입니다. p35는 주로 뇌에서 발현되는 반면 p25로 알려진 단축형은 다른 조직에서 발견될 수 있으며, p25가 축적될 때 신경 퇴행성 질환에 영향을 미칠 수 있습니다. p35(4G11) 항체의 특이성과 기능성은 정상 상태와 병리학적인 상태 모두에서 Cdk5와 조절 메커니즘의 복잡한 역할을 연구하는 연구자들에게 매우 중요한 도구입니다.

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p35 항체 (4G11)

sc-293184
100 µg/ml
$316.00

培養細胞にてCDK5R1の発現についてWestern blottingでの解析,あるいは,剖検脳組織での局在をみるために免疫染色を計画しております.CDK5R1とp35の異同が分からず,混乱しております.NCBIのCDK5R1に関するサイトでは,「also known as」としてp35も記載されておりますが,同一と考えればよろしいのでしょうか?あるいは,どの抗体使用することが推奨されるでしょうか?

Asked by: himawari
質問頂き、誠にありがとうございます。CDK5R1はp35の別名である、この遺伝子によってコードされるタンパク質(p35)は、サイクリン依存性キナーゼ5(CDK5)のニューロン特異的活性化因子であり、CDK5の活性化は中枢神経系の適切な発達に必要である。https://www.uniprot.org/uniprotkb/Q15078/entry#names_and_taxonomy CDK5R1 is another name for p35.The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. https://www.uniprot.org/uniprotkb/Q15078/entry#names_and_taxonomy
Answered by: Jiajun Z
Date published: 2023-12-25

Hi! Does this antibody recognize p25, the proteolytic product of p35?

Asked by: Wayne Jiang
Thank you for your question. According to Uniprot, (http://www.uniprot.org/uniprot/Q15078#ptm_processing), the cleaved p25 form of p35 corresponds to amino acids 99-307. This mouse monoclonal antibody, p35 (4G11): sc-293184, is raised against amino acids 208-307 of human p35. Therefore, the antibody does have an epitope that should recognize the cleaved p25 form. We have not confirmed this experimentally.
Answered by: Technical Support
Date published: 2017-05-17

I am using p35 (4G11): sc-293184 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jenniferc
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
  • y_2025, m_9, d_5, h_6CST
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Rated 2 out of 5 by from Not great at detecting endogenous proteinWorks at detecting overexpressed p35 on western blot pretty well, but not great when looking at endogenous p35- lots of other background bands. WB 1:1000 Overexpressed p35 in 293 cells or endogenous p35 from embryonic mouse brain (cortex) lysates.
Date published: 2017-11-18
Rated 3 out of 5 by from GoodOk, `36 kDa in 10% PAGE Used at an dilution of 1:250. Left lane loaded with protein standard marker 250, 130, 100, 55, 35, 25 kDa bands. Loaded with 35ug.
Date published: 2017-10-27
Rated 4 out of 5 by from works good for western blotwork well in WB at 1:1000,mouse.Strip is clear and the location is very accurate
Date published: 2017-10-08
Rated 4 out of 5 by from works good for western blotI got the free sample the last week and I got clear bands for western blot using lysates from cultured neurons and mouse brains. In addition, It successfully detects p35 and p25 in cell lysates. My PVDF membrane is probed with this antibody in 3% milk/TBST with 1:500 dilution at 4oC for 1 overnight.
Date published: 2017-09-19
Rated 4 out of 5 by from works greatlyI got the sample of this antibody and tested with breast cancer cell line with 1:1000. It works great with specific band at the right size.
Date published: 2017-08-20
Rated 4 out of 5 by from work for wbthe band is clear ,but the concentration is not high ,with 1:300
Date published: 2017-08-13
Rated 2 out of 5 by from good but need high concentrationI got the free sample and used for 15 ug of rat brain homogenate, with 1 to 200 dilution and enhanced ECL for 2 min we got the good signal.
Date published: 2017-08-10
Rated 1 out of 5 by from This p35 (4G11) antibody didn't work on WB.I got the p35 (4G11) antibody a few days ago and used the recommended secondary antibody sc-516102 to get the signal of LNCaP protein lysate by western blotting. There is nothing. No band (signal) on PVDF membrane. =>Primary antibody dilution is 1:1500~1:750, tried and got nothing. =>Plus secondary antibody dilution from 1:10000 to 1:5000, also got nothing. I got normal signal one the same target and same cell lysate and same WB method, the p35 (c-19) sc-820 works fine and perfect. Very sad!!
Date published: 2017-06-15
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p35 항체 (4G11) is rated 3.0 out of 5 by 8.
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