Date published: 2025-9-8

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p14ARF 항체 (DCS-240): sc-53639

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데이터 시트
  • p14ARF 항체(DCS-240) 는 마우스 monoclonal IgG1κ, 14간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human p14 ARF의 N-terminus 세포질 영역 내의 아미노산 사이의 에피토프 매핑에 특이적
  • p14ARF 항체 (DCS-240)는 WB, IP and IF으로 human유래의 p14 ARF 를 감지하는 데에 추천한다 .
  • 항-p14ARF 항체(DCS-240)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • p14ARF (DCS-240): sc-53639무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP 는 p14ARF 항체 (DCS-240) for WB applications. 이 시약은 이제 p14ARF 항체 (DCS-240)와 함께 번들로 제공됩니다(아래 주문 정보 참조).

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p14 ARF 항체(DCS-240)는 웨스턴 블롯팅(WB), 면역 침전(IP) 및 면역 형광(IF)으로 인간 유래의 p14 ARF를 검출하는 마우스 단일 클론 IgG1 카파 경쇄 항체입니다. p14 ARF 단일 클론 항체(DCS-240)는 아가로스, 홀스래디쉬 퍼옥시다제(HRP), 피코에리스린(PE), 플루오레세인 이소티오시안산염(FITC) 및 다중 Alexa Fluor® 컨쥬게이트 등 비접합 및 다양한 컨쥬게이트 형태로 제공됩니다. p14 ARF(DCS-240) 항체는 세포의 완전성을 유지하고 통제되지 않은 세포 증식을 방지하는 데 필수적인 종양 억제제 역할을 하여 세포 주기 조절에 중요한 역할을 하는 단백질을 인식합니다. p14 ARF는 p16 단백질을 생성하는 CDKN2A 유전자에 의해 암호화되며, p53 종양 억제 경로의 음성 조절자인 MDM2와 상호 작용합니다. MDM2에 결합함으로써 p14 ARF는 p53 수준을 안정화하여 p21의 발현을 증가시키고 이후 G1 및 G2/M 단계에서 세포 주기를 정지시킵니다. 이 조절 메커니즘은 손상된 세포가 계속 분열하지 않기 때문에 종양 형성을 예방하는 데 필수적입니다. 이러한 중요한 경로를 조절하는 p14 ARF의 능력은 세포 주기 조절 및 종양 억제에 초점을 맞춘 연구에서 항-p14 ARF 항체(DCS-240)의 중요성을 강조합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

p14ARF 항체 (DCS-240)

sc-53639
200 µg/ml
$316.00

p14ARF (DCS-240): m-IgG Fc BP-HRP 번들

sc-526839
200 µg Ab; 10 µg BP
$354.00

p14ARF (DCS-240): m-IgG1 BP-HRP 번들

sc-532212
200 µg Ab; 20 µg BP
$354.00

p14ARF 항체 (DCS-240) AC

sc-53639 AC
500 µg/ml, 25% agarose
$416.00

p14ARF 항체 (DCS-240) HRP

sc-53639 HRP
200 µg/ml
$316.00

p14ARF 항체 (DCS-240) FITC

sc-53639 FITC
200 µg/ml
$330.00

p14ARF 항체 (DCS-240) PE

sc-53639 PE
200 µg/ml
$343.00

p14ARF 항체 (DCS-240) Alexa Fluor® 488

sc-53639 AF488
200 µg/ml
$357.00

p14ARF 항체 (DCS-240) Alexa Fluor® 546

sc-53639 AF546
200 µg/ml
$357.00

p14ARF 항체 (DCS-240) Alexa Fluor® 594

sc-53639 AF594
200 µg/ml
$357.00

p14ARF 항체 (DCS-240) Alexa Fluor® 647

sc-53639 AF647
200 µg/ml
$357.00

p14ARF 항체 (DCS-240) Alexa Fluor® 680

sc-53639 AF680
200 µg/ml
$357.00

p14ARF 항체 (DCS-240) Alexa Fluor® 790

sc-53639 AF790
200 µg/ml
$357.00

I would like to know if p14ARF antibody (DCS-240) is better diluted in milk or BSA 5%. Thank you!

Asked by: Gpb27
Thank you for your question. Ideal blocking conditions are dependent on a variety of different factors. If your western blots show a lot of background, milk will provide a broader blocking effect, while a BSA-based blocking buffer will prevent overblocking of weaker signals.
Answered by: Tech Support Europe
Date published: 2020-12-01

Is this antibody specific for p14 instead of p16 or can it detect both p14 and p16 from human cells? Thank you!

Asked by: Mai Xu
Thank you for your question. This monoclonal antibody is specific for p14 ARF of human origin.
Answered by: Technical Support
Date published: 2019-06-16

I would like to use p14 ARF Antibody (DCS-240): sc-53639 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-53639 is not available directly conjugated, but we have an alternative p14 ARF antibody (sc-53392) that is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 5
Date published: 2017-02-08
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Rated 4 out of 5 by from Work for In cell westernI only tried this antibody for in cell western and it works fine in dilution 1:400.
Date published: 2018-01-30
Rated 5 out of 5 by from Very popular choice!This monoclonal p14 ARF Antibody (DCS-240) is a very popular choice, especially for Western blotting of human samples, as indicated by the many product citations for this clone.
Date published: 2017-01-26
Rated 5 out of 5 by from OKAntibody was published for use in IP and WB on human Hela and H1299 Cells. the wb:1:500 is OK
Date published: 2016-12-07
Rated 5 out of 5 by from Antibody was published for use in IP andAntibody was published for use in IP and WB on human 293T and H1299 cell - SCBT Publication Review
Date published: 2015-04-27
Rated 5 out of 5 by from Nice Western blot data obtained in BJAB wholeNice Western blot data obtained in BJAB whole cell lysate. -SCBT QC
Date published: 2015-03-29
Rated 5 out of 5 by from WB publishable data with human stable fibroblastWB publishable data with human stable fibroblast cell lines - SCBT Publication Review
Date published: 2015-01-27
Rated 5 out of 5 by from Good data published with this antibody inGood data published with this antibody in WB on immortalized human fibroblast cell line (hTeRT-BJ1 cells) - SCBT Publication Review
Date published: 2015-01-05
Rated 5 out of 5 by from Publishable WB in human MDAPublishable WB in human MDA-MB-231 cells and hTERT-BJ1 cells -SCBT Publication Review
Date published: 2013-10-03
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p14ARF 항체 (DCS-240) is rated 4.9 out of 5 by 8.
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