p-VASP Antibody (F-3): sc-365564

p-VASP Antibody (F-3) is a mouse monoclonal IgM antibody that detects phosphorylated VASP, specifically Ser 157 phosphorylated VASP, in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). p-VASP (F-3) antibody is available in a non-conjugated form, allowing for versatile experimental use. VASP (vasodilator-stimulated phosphoprotein) plays a crucial role in maintaining cellular architecture by interacting with actin filaments, which is essential for various cellular processes, including cell migration and adhesion. This interaction is particularly important in the context of the Wiskott-Aldrich syndrome, where mutations in the WASP gene lead to significant immune deficiencies and other complications. VASP is phosphorylated at serine 157 by cAMP and cGMP-dependent kinases, which regulates VASP activity and localization to focal adhesions and microfilaments, thereby influencing cytoskeletal dynamics. VASP is also phosphorylated at serine 239 by cGMP-dependent protein kinase, further modulating VASP function in cellular signaling pathways. Anti-p-VASP antibody (F-3) specifically recognizes phosphorylated VASP, making this antibody a valuable tool for studying VASP′s role in health and disease.

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p-VASP Antibody (F-3) References:

1. Association of VASP with TRPC4 in PKG-mediated inhibition of the store-operated calcium response in mesangial cells.  |  Wang, X., et al. 2007. Am J Physiol Renal Physiol. 293: F1768-76. PMID: 17913834
2. Differential phosphoproteome profiling reveals a functional role for VASP in Helicobacter pylori-induced cytoskeleton turnover in gastric epithelial cells.  |  Knauer, O., et al. 2008. Cell Microbiol. 10: 2285-96. PMID: 18637808
3. Promotion of PDGF-induced endothelial cell migration by phosphorylated VASP depends on PKA anchoring via AKAP.  |  Zhang, D., et al. 2010. Mol Cell Biochem. 335: 1-11. PMID: 19711176
4. Thrombin and collagen induce a feedback inhibitory signaling pathway in platelets involving dissociation of the catalytic subunit of protein kinase A from an NFkappaB-IkappaB complex.  |  Gambaryan, S., et al. 2010. J Biol Chem. 285: 18352-63. PMID: 20356841
5. Herpes simplex virus type 1 entry into epithelial MDCKII cells: role of VASP activities.  |  Jaeger, V., et al. 2010. J Gen Virol. 91: 2152-7. PMID: 20463151
6. Decreased VASP phosphorylation in platelets of male and female smokers of young age.  |  Assinger, A., et al. 2010. Platelets. 21: 596-603. PMID: 20822337
7. Tributyltin induces disruption of microfilament in HL7702 cells via MAPK-mediated hyperphosphorylation of VASP.  |  Tu, WW., et al. 2016. Environ Toxicol. 31: 1530-1538. PMID: 26018654
8. Guanylin-Guanylyl cyclase-C signaling in macrophages regulates mesenteric fat inflammation induced by high-fat diet.  |  Hasegawa, K., et al. 2015. Endocr J. 62: 939-47. PMID: 26249840
9. Shen-Fu Decoction could ameliorate intestinal permeability by regulating the intestinal expression of tight junction proteins and p-VASP in septic rats.  |  Liu, F., et al. 2021. J Ethnopharmacol. 268: 113562. PMID: 33217519
10. Endothelium-dependent phosphorylation of vasodilator-stimulated protein in platelets during coronary passage.  |  Pohl, U., et al. 1994. Am J Physiol. 266: H606-12. PMID: 8141362

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-VASP Antibody (F-3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-p-VASP Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-VASP Antibody (F-3) Specifications

Alternate Product Name: phosporylated VASP

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: Ser 157

Epitope Target: VASP

Epitope Species: human

Product Reactivity/Detection: Ser 157 phosphorylated VASP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for p-VASP Antibody (F-3): P: Blocking Peptide

The preferred VASP antibody is VASP Antibody (A-11): sc-46668.