p-Shc Antibody (73.Tyr 349/350): sc-293148

p-Shc Antibody (73.Tyr 349/350) is a mouse monoclonal IgG1 antibody that detects p-Shc in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). p-Shc (73.Tyr 349/350) antibody recognizes a protein that plays a crucial role in cellular signaling pathways, particularly in growth factor receptor activation and downstream signaling cascades. p-Shc is primarily located in the cytoplasm and participates in signal transduction from activated receptors to various intracellular pathways, including the Ras/MAPK pathway, essential for cell proliferation and differentiation. Phosphorylation of specific tyrosine residues on p-Shc, particularly Tyr 349 and Tyr 350, enables interaction with the Grb2-Sos complex, facilitating Ras activation. This interaction links receptor tyrosine kinases to MAPK activation, a pathway often dysregulated in cancer. p-Shc′s role in mediating these signaling events underscores its importance in both normal cellular function and disease states, making anti-p-Shc antibody (73.Tyr 349/350) an invaluable tool for researchers studying signal transduction and its implications in various biological processes.

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p-Shc Antibody (73.Tyr 349/350) References:

1. Signal transduction through tyrosine-phosphorylated C-terminal fragments of amyloid precursor protein via an enhanced interaction with Shc/Grb2 adaptor proteins in reactive astrocytes of Alzheimer's disease brain.  |  Russo, C., et al. 2002. J Biol Chem. 277: 35282-8. PMID: 12084708
2. Signal transduction through tyrosine-phosphorylated carboxy-terminal fragments of APP via an enhanced interaction with Shc/Grb2 adaptor proteins in reactive astrocytes of Alzheimer's disease brain.  |  Russo, C., et al. 2002. Ann N Y Acad Sci. 973: 323-33. PMID: 12485888
3. Role of SHPS-1 in the regulation of insulin-like growth factor I-stimulated Shc and mitogen-activated protein kinase activation in vascular smooth muscle cells.  |  Ling, Y., et al. 2005. Mol Biol Cell. 16: 3353-64. PMID: 15888547
4. Adaptor protein Shc acts as an immune-regulator for the LPS-stimulated maturation of bone marrow-derived dendritic cells.  |  Chen, KD., et al. 2011. BMC Immunol. 12: 32. PMID: 21612602
5. Phospho-Tyrosine(s) vs. Phosphatidylinositol Binding in Shc Mediated Integrin Signaling.  |  Lin, X. and Vinogradova, O. 2015. Am J Mol Biol. 5: 17-31. PMID: 25893141
6. Specific motifs recognized by the SH2 domains of Csk, 3BP2, fps/fes, GRB-2, HCP, SHC, Syk, and Vav.  |  Songyang, Z., et al. 1994. Mol Cell Biol. 14: 2777-85. PMID: 7511210
7. A family of Shc related proteins with conserved PTB, CH1 and SH2 regions.  |  Pelicci, G., et al. 1996. Oncogene. 13: 633-41. PMID: 8760305
8. Distinct mechanisms mediate SHC association with the activated and resting B cell antigen receptor.  |  D'Ambrosio, D., et al. 1996. Eur J Immunol. 26: 1960-5. PMID: 8765045
9. Tyrosine 474 of ZAP-70 is required for association with the Shc adaptor and for T-cell antigen receptor-dependent gene activation.  |  Pacini, S., et al. 1998. J Biol Chem. 273: 20487-93. PMID: 9685404
10. Gads is a novel SH2 and SH3 domain-containing adaptor protein that binds to tyrosine-phosphorylated Shc.  |  Liu, SK. and McGlade, CJ. 1998. Oncogene. 17: 3073-82. PMID: 9872323

The preferred Shc antibody is Shc Antibody (PG-797): sc-967.