human origin의 OSX에서 에 가까운 172-268 아미노산을 항원으로 사용하였습니다.
안티-OSX 항체 (F-3)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 OSX 를 감지하는 데에 추천한다.
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-OSX 항체 (F-3)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-393325 X, 200 µg/0.1 ml)이 있습니다.
m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for OSX Antibody (F-3) for WB applications. These reagents are now offered in bundles with OSX Antibody (F-3) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
OSX Antibody (F-3) is a high quality monoclonal OSX antibody (also designated Sp7 antibody or Osterix antibody) suitable for the detection of the OSX protein of mouse, rat and human origin. OSX Antibody (F-3) is available as both the non-conjugated anti-OSX antibody form, as well as multiple conjugated forms of anti-OSX antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Osterix (OSX) is a zinc finger-containing transcriptional activator that is distinctly expressed in all developing bones and is important for osteoblast differentiation. In particular, OSX is implicated in the differentiation of osteo-blasts, which are the specialized cells of bone formation. OSX is a nuclear protein that binds to GC box promoters elements and activates mRNA synthesis from genes containing functional recognition sites. The periosteal and mesenchymal cells of the membranous skeletal elements of OSX- mice fail to differentiate into osteoblasts. Subsequently, the mesenchymal cells of OSX- mice fail to deposit bone matrix and do not form bone. Cox-2 deficiency correlates with a decrease in OSX expression, suggesting that Cox-2 may induce OSX to mediate skeletal repair.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences