OSX 항체 (F-3) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 OSX을 검출할것을 권장합니다.

OSX 항체 (F-3): sc-393325

OSX 항체 (F-3) is rated 4.5 out of 5 by 13.
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데이터 시트

    • 안티-OSX 항체 F-3 는 마우스 monoclonal IgG1 κ OSX 항체, 44간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 OSX에서 에 가까운 172-268 아미노산을 항원으로 사용하였습니다.
    • 안티-OSX 항체 (F-3)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 OSX 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-OSX 항체 (F-3)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-393325 X, 200 µg/0.1 ml)이 있습니다.
    • OSX (F-3): sc-393325무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for OSX Antibody (F-3) for WB applications. These reagents are now offered in bundles with OSX Antibody (F-3) (see ordering information below).
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Which secondary antibodies do you recommend for IF staining in mouse tissues?

Asked by: jackie34
Thank you for your question. You can find our anti-mouse secondary binding proteins by following this link: https://www.scbt.com/browse/Secondary-Antibodies/_/N-1hoaxk4 OSX Antibody (F-3): sc-393325 is a kappa light chain antibody, so any of our kapppa binding proteins such as m-IgGκ BP-CFL 488 sc-516176 would be suitable.
Answered by: Tech Support Europe
Date published: 2019-11-22

Are there any issues with non-specific staining when using this mouse monoclonal antibody on mouse tissue samples in IF or IHC?

Asked by: AbPolly
Thank you for your question. This antibody is recommended for use with mouse samples in IF and IHC. To eliminate any potential issues with non-specific binding of the anti-mouse secondary, we recommend using the directly conjugated version of the primary. This antibody is available conjugated to PE, FITC, AF488 and AF647.
Answered by: Technical Support 15
Date published: 2020-07-26
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Rated 4 out of 5 by from Good antibody The band is clear but not so strong. You need a good ECL.
Date published: 2018-04-06
Rated 4 out of 5 by from One band with high background I had a trial sample for this antibody, and got one band (~41kDa) in mouse periodontal stem cell lysate. Maybe because of the homology, there are several background bands.
Date published: 2017-08-21
Rated 4 out of 5 by from seems good It worked well for IHC in mouse femur section though the stability of this product still needs to be proved.
Date published: 2017-03-08
Rated 4 out of 5 by from good quanlity We observed two bands of OSX in WB. Dilution of primary antibody is 1: 500
Date published: 2017-03-06
Rated 5 out of 5 by from Good Antibody OSX Antibody Neurogenin 3 antibody could be performed under isothermal conditions, its specificity was good and the sensitivity. We were be quite content with the antibody.
Date published: 2017-02-13
Rated 5 out of 5 by from Between samples of rat bone marrow mesenchymal ste We observed the work main antibody dilution was 1:500. Between samples of rat bone marrow mesenchymal stem cells.
Date published: 2017-02-09
Rated 5 out of 5 by from Suitable for immunofluorescence We used sc-393325 to make immunofluorescence, and the staining pattern was very strong.
Date published: 2017-02-07
Rated 5 out of 5 by from Positive results Observed positive WB results with OSX Antibody (F-3)
Date published: 2017-01-18
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