OSBP 항체 (H-5) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 OSBP을 검출할것을 권장합니다.

OSBP 항체 (H-5): sc-515683

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데이터 시트

    • OSBP 항체(H-5) 는 마우스 monoclonal IgG1(kappa light chain) 이며 200 µg/ml으로 제공합니다.
    • 항원결정부는 human origin의 OSBP의 내부에 위치한 778-793의 아미노산에 해당됩니다.
    • WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 OSBP을 검출할것을 권장합니다.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for OSBP Antibody (H-5) for WB applications. These reagents are now offered in bundles with OSBP Antibody (H-5) (see ordering information below).
loading

How much sample do you recommend loading on the gel for Western blot detection using OSBP (H-5): sc-515683?

Asked by: Cweed
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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