osteopontin/OPN/SPP1 항체 (AKm2A1) WB, IP, IF와 IHC(P)으로 mouse, rat와 human origin의 OPN을 검출할것을 권장합니다.

osteopontin/OPN/SPP1 항체 (AKm2A1): sc-21742

osteopontin/OPN/SPP1 항체 (AKm2A1) is rated 4.4 out of 5 by 26.
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데이터 시트

    • 안티-osteopontin/OPN/SPP1 항체 AKm2A1 는 마우스 monoclonal IgG1 κ osteopontin/OPN/SPP1 항체, 310간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • raised against mouse recombinant osteopontin
    • 안티-osteopontin/OPN/SPP1 항체 (AKm2A1)는 WB, IP, IF and IHC(P)으로 mouse, rat and human유래의 OPN 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-osteopontin/OPN/SPP1 항체 (AKm2A1)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • osteopontin/OPN/SPP1 (AKm2A1): sc-21742무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for osteopontin/OPN/SPP1 Antibody (AKm2A1) for WB and IHC(P) applications. These reagents are now offered in bundles with osteopontin/OPN/SPP1 Antibody (AKm2A1) (see ordering information below).
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primary mouse monoclonal antibody to detect mouse protein-that will generate much background after anti-mouse 2nd antibody. Do you have specail prtotocol to avoid the effect? I need to do IHC with ABC method for mouse sample. Thank you! Kai

Asked by: Kal69
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Anonymous
Date published: 2021-12-15

Hello, Does this antibody recognize the N-terminus celaved fragment of OPN, the C-terminus fragment or both?

Asked by: Chris G
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions
Answered by: Blake J
Date published: 2021-10-11

Can I use this antibody for ip injection to neutralize the OPN in mice for long-term study?

Asked by: Yan T
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

How much would it be if I want to order 25 mg, 50 mg and 100 mg of this antibody for animal treatment (long-term)?

Asked by: Yan T
Our antibodies cannot be used for diagnostic or therapeutic purposes.
Answered by: Tech Sales
Date published: 2021-06-25

Does this antibody work well using IHC on rat samples?

Asked by: SR79
Thank you for your question. Yes, this antibody is recommended and warrantied for IHC on rat samples.
Answered by: Tech Service
Date published: 2020-01-10

I want to use just a few of this antibody ,so how can i extend the period of validity

Asked by: Lucia1985
Thank you for your question. We recommend aliquoting and storing this antibody at 4C for long-term storage. Please contact Technical Service if you require further support.
Answered by: Technical Support
Date published: 2018-12-13

How long is the period of validity?Once I open it, How long should I use it up so that it is at the period of validity.

Asked by: halen
Thank you very much for the question. We offer a product warranty for up to one year starting from the shipping date. If you have further questions please contact our Technical Service department.
Answered by: Technical Support Europe
Date published: 2018-01-19

What is the suggested positive control for this marker in IHC? Large intestine sections were used as a positive control in some researches, is this appropriate ? 

Asked by: Rola
Thank you for your inquiry and your interest in this product. We have tested this antibody in IHC and show several immunoperoxidase stainings on different formalin fixed, paraffin-embedded human tissues including kidney, gall bladder, placenta tissue and bone marrow. We have not tested it on large intestine sections. Please contact our European Support Office in Germany directly for any further questions you may have. You can reach us by phone at: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com We are happy to assist you.
Answered by: Technical Support Europe
Date published: 2017-12-27
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Rated 5 out of 5 by from SK030204V100 This antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2022-01-05
Rated 5 out of 5 by from 功能很出色 我一个月前购买了该抗体,用得很好,老板很满意,还要多写几个字,其实我也不知道写啥好。为了多写一些。 还少啊
Date published: 2022-01-05
Rated 5 out of 5 by from Very good antibody Works as we expected. Tested by immunofluorescence. Great
Date published: 2018-12-18
Rated 5 out of 5 by from Works well for IHC This antibody was generously given to me by the trials program. I found that it works well for IHC at a dilution of 1:50 to 1:250 in whole mount of retinal tissue. It works better with PFA than Methanol fixation.
Date published: 2018-03-14
Rated 5 out of 5 by from 抗体很好用,条带特别单一。 申请了试用装,抗体特别好用,特异性很好。从开始用到现在已经有三个月的时间,一直用的是开始配的抗体,用了五六次,抗体效价很高。
Date published: 2018-02-07
Rated 5 out of 5 by from good anti-body This product is used in broiler pulmonary artery smooth muscle cells, the effect is good, 1: 50 dilution
Date published: 2018-01-10
Rated 3 out of 5 by from two bands were observed Mouse liver, 20ug protein, two bands were observed, the upper one was between 55 and 40 kDa, and the lower one was below 40kda, i am not sure whether the upper one is OPN?
Date published: 2018-01-09
Rated 1 out of 5 by from Does not work for mouse aorta samples I did WB for mouse aorta samples, only 55kd and 25kd two unspecific bands were obtained, I guess they are IgG heavy chain and light chain. No expected bands
Date published: 2017-12-14
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