NGF Double Nickase Plasmid (m): sc-421889-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NGF Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NGF Double Nickase Plasmid (m) and NGF Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ngf. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NGF Antibody (NGF30): sc-32300
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NGF Double Nickase Plasmid (m) | sc-421889-NIC | 20 µg | $410.00 | |||
NGF Double Nickase Plasmid (m2) | sc-421889-NIC-2 | 20 µg | $410.00 |
Ngf encodes nerve growth factor (NGF), a secreted neurotrophin essential for the survival, differentiation, and maintenance of sympathetic and sensory neurons during development and in adult tissues. NGF signals primarily through NTRK1/TrkA and p75NTR to regulate MAPK/ERK, PI3K–AKT, and PLCγ pathways, linking ligand availability to neurite outgrowth, synaptic plasticity, and neuronal stress responses. In mouse models, altered NGF signaling is associated with changes in nociceptor sensitization, neuroimmune interactions, and peripheral innervation dynamics relevant to inflammatory and neuropathic pain biology. NGF also influences non-neuronal processes including mast cell activity and tissue remodeling, making Ngf a useful node for studying neurotrophin-dependent crosstalk in disease-relevant microenvironments.
NGF Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ngf locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ngf. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ngf function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ngf-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.