Date published: 2025-9-9
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Neurofibromin Antibody (McNFn27a) - Immunohistochemistry - Image 284765
뉴로피브로민 항체(McNFn27a): sc-20017. 간 정현파의 세포질 염색을 보여주는 포르말린 고정, 파라핀 매립 인간 간 조직의 면역 산화 효소 염색.
Neurofibromin Antibody (McNFn27a) - Immunohistochemistry - Image 284765
뉴로피브로민 항체(McNFn27a): sc-20017. 간 정현파의 세포질 염색을 보여주는 포르말린 고정, 파라핀 매립 인간 간 조직의 면역 산화 효소 염색.
Neurofibromin Antibody (McNFn27a) - Western Blotting - Image 438
뉴로피브로민 항체(McNFn27a): sc-20017. NF1 녹아웃(A), 쥐(B) 및 인간(C) 슈반 세포에서 뉴로피브로민 발현의 웨스턴 블롯 분석. 플로리다 대학교 의과대학 데이비드 뮤어 박사가 친절하게 제공.
뉴로피브로민 항체(McNFn27a): sc-20017. 간 정현파의 세포질 염색을 보여주는 포르말린 고정, 파라핀 매립 인간 간 조직의 면역 산화 효소 염색.

Neurofibromin 항체 (McNFn27a): sc-20017

3.3(3)
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데이터 시트
  • Neurofibromin 항체(McNFn27a) 는 마우스 monoclonal IgG1κ, 4간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human의 Neurofibromin에서 27-41 아미노산에 해당하는 N-terminal 영역을 대상으로 생성됨
  • WB, IP, IF와 IHC(P)으로 mouse, rat와 human origin의 Neurofibromin을 검출할것을 권장합니다.
  • See Neurofibromin (H-12): sc-376886 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 와 m-IgGκ BP-HRP 는 Neurofibromin 항체 (McNFn27a) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은Neurofibromin 항체(McNFn27a) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

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신경섬유종증 제1형 (NF1) 또는 폰 레킹하우젠 신경섬유종증은 인간의 가장 흔한 상염색체 우성 질환 중 하나이다. 초기 연계 분석은 NF1 유전자를 17번 염색체에 매핑했다. 예측된 NF1 전사체는 NF1-GAP 관련 단백질 (NF1GRP)로도 명명된 2,818개의 아미노산 단백질 뉴로피브로민을 인코딩한다. 시퀀스 분석에 의해, 뉴로피브로민과 Ras GAP 유전자 계열의 구성원들의 작은 영역 내에서 유사성이 입증되었다. 기능적으로, 뉴로피브로민은 GAP 단백질과 더욱 유사하도록 효모의 RAS-GAP 가수분해 및 기능적 보완을 포함하는 생화학적 분석에 의해 보여졌다. 뉴로피브로민 단백질은 뇌, 폐, 간, 신장, 비장, 근육 및 결장을 포함한 광범위한 세포주 및 조직에서 비교적 일정한 수준으로 발현된다. 뉴로피브로민의 기능에 대해서는 거의 알려져 있지 않지만, GTPase 활성을 갖는 단백질의 촉매 도메인과의 상동성은 뉴로피브로민이 Ras 단백질과도 생체 내에서 상호작용할 수 있음을 시사한다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

Neurofibromin 항체 (McNFn27a)

sc-20017
200 µg/ml
$316.00

Neurofibromin (McNFn27a): m-IgG Fc BP-HRP 번들

sc-536640
200 µg Ab; 10 µg BP
$354.00

Neurofibromin (McNFn27a): m-IgGκ BP-HRP 번들

sc-533797
200 µg Ab; 40 µg BP
$354.00

Neurofibromin (McNFn27a): m-IgG1 BP-HRP 번들

sc-544725
200 µg Ab; 20 µg BP
$354.00

I am using Neurofibromin (McNFn27a): sc-20017 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from This antibody genuinely *does* work (even on WB)Hi. I am first author of one of the cited publications for this antibody (at least my pub still listed as 08/2025; Kahen, E. et al.) and I noticed someone else said they could not get signal on WB in their hands. I want to reassure that you really can get great WB's with this antibody for any human cell line, but it is a labor of love. Neurofibromin is a fairly large protein as you likely know, (typically considered 240 kd) often running somewhere around 210-240 kda range on a gel per MW markers. As I wrote in my methods, I had to use a tris-acetate gradient gel of 3-8% to capture NF1 and its putative isoform explicitly (Very painful gel to deal with; many gels were broken during troubleshooting). Getting the loading control was tricky because tubulin would run around the same range as a non-specific band. Alpha-actinin ran *very close* to a non-specific band so was not "publication worthy" but was good for testing, and was used, and others might benefit from this using alpha-actinin for loading control for NF1 if new antibodies more specific. I had to find perfect timing to be able to capture proper *actin* right at the bottom of the gel, which has far more specific WB antibodies for pub-worthy images. Note: Wet transfer 100% necessary to get all the NF1. Dry transfer would either over-transfer the actin or (most often) under-transfer the NF1. Very difficult to get sufficient NF1 on a membrane via dry-transfer without losing other bands for controls (and I was also really trying to get RAS on same gel). Honestly I think I legit tried dry transfer for a hilarious amount of time once and when I stained the gel out of curiosity, there was *still* so much protein in the NF1 region. I really tried many times with dry transfer- wet transfer only way to get anything useful. Then, I actually blocked membranes *overnight* in Odyssey blocking buffer. This Santa-Cruz NF1 antibody was then used at highest concentration recommended by manufacturer, again in Odyssey blocking buffer *overnight* (4degC on shaker). Very thorough washing in TBST (and I think I actually preferred lower tween 20 % but 10 min washes). I really wanted clean crisp neurofibromin showing two bands, as I did. And ultimately, I was able to get the result I wanted and reproduce it (though actin could really have looked a little prettier, but quantified accurately overall.) I suppose we should really be this detailed in our methods, explaining these sorts of things, but we tend not to be. Nonetheless, what I've described might help someone working with this antibody. Feel free to reach out via email and I will try to remember my best other than what I've written here.
Date published: 2025-08-02
Rated 1 out of 5 by from Not working for WB in our handsessentially no specific bands, nor non-specific bands.
Date published: 2019-05-29
Rated 4 out of 5 by from WB: Worked well in mouse spleen samplesWB: Worked well in mouse spleen samples. -SCBT Publication Review
Date published: 2015-06-18
Rated 5 out of 5 by from Published data for IF in human Schwann cellsPublished data for IF in human Schwann cells and CCF cells. -SCBT Publication Review
Date published: 2013-05-05
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Neurofibromin 항체 (McNFn27a) is rated 3.8 out of 5 by 4.
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